Koutroulis, Andreas; Menon, Nandita; Kapralos, Vasileios; Ørstavik, Dag; Pain, Maria; Valen, Håkon; Camilleri, Josette; Kishen, Anil; Sunde, Pia Titterud
Investigation of the effect of addition/replacement of bioactive glass to hydraulic calcium silicate cement Journal Article
In: Dental Materials, vol. 41, no. 9, pp. 1067 – 1079, 2025, ISSN: 01095641, (Cited by: 0).
@article{Koutroulis20251067,
title = {Investigation of the effect of addition/replacement of bioactive glass to hydraulic calcium silicate cement},
author = {Andreas Koutroulis and Nandita Menon and Vasileios Kapralos and Dag Ørstavik and Maria Pain and Håkon Valen and Josette Camilleri and Anil Kishen and Pia Titterud Sunde},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-105009098552&doi=10.1016%2fj.dental.2025.06.018&partnerID=40&md5=b35aed774829c9edd8db9af3e8701828},
doi = {10.1016/j.dental.2025.06.018},
issn = {01095641},
year = {2025},
date = {2025-01-01},
journal = {Dental Materials},
volume = {41},
number = {9},
pages = {1067 – 1079},
publisher = {Elsevier Inc.},
abstract = {Objectives: Assess the effect of addition or replacement of bioactive glass (BG) on the physico-chemical, antibacterial and biological properties of radiopacified tricalcium silicate cement (TCS). Methods: Four materials were tested: An experimental cement with 80 % TCS, 20 % zirconium oxide (TZ-base); two modified versions of TZ-base incorporating 20 % BG 45S5 either as TCS-replacement (TZ-bg-R) or addition (TZ-bg-A); and Biodentine (Septodont). Setting time was assessed using ISO 6876:2012 method. Materials were immersed in water or Dulbecco's modified eagle medium with 10 % fetal bovine serum (DMEM-sup). After seven days, water sorption and solubility were assessed using ISO 4049:2019 methods; water uptake and porosity were also calculated; leachate alkalinity and calcium release were assessed with a pH meter and inductively coupled plasma respectively; specimens were characterized with scanning electron microscopy, energy dispersive X-ray and X-ray diffraction analysis. At one-day, antibacterial properties of material surfaces and leachates were tested against Enterococcus faecalis and Staphylococcus epidermidis biofilms; leachate cytotoxicity and cytokine release were assessed with the 3-(4,5 dimethylthiazolyl-2-yl)-2,5-diphenyl tetrazolium bromide assay and an enzyme-linked immunosorbent assay. Regression models were fitted for all dependent variables. Results: Compared to TZ-base, the BG-containing materials had overall increased water sorption, water uptake and porosity; decreased solubility in DMEM-sup; lower calcium release; and generally similar pH, antibacterial activity, cytotoxicity and cytokine release. All experimental cements were more cytotoxic than Biodentine, but the latter caused higher cytokine release. Significance: The BG-incorporation whether added or replacing the cement decreased the solubility in clinically relevant media and did not deteriorate the antimicrobial and biological properties of the materials. © 2025 The Authors},
note = {Cited by: 0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Objectives: Assess the effect of addition or replacement of bioactive glass (BG) on the physico-chemical, antibacterial and biological properties of radiopacified tricalcium silicate cement (TCS). Methods: Four materials were tested: An experimental cement with 80 % TCS, 20 % zirconium oxide (TZ-base); two modified versions of TZ-base incorporating 20 % BG 45S5 either as TCS-replacement (TZ-bg-R) or addition (TZ-bg-A); and Biodentine (Septodont). Setting time was assessed using ISO 6876:2012 method. Materials were immersed in water or Dulbecco's modified eagle medium with 10 % fetal bovine serum (DMEM-sup). After seven days, water sorption and solubility were assessed using ISO 4049:2019 methods; water uptake and porosity were also calculated; leachate alkalinity and calcium release were assessed with a pH meter and inductively coupled plasma respectively; specimens were characterized with scanning electron microscopy, energy dispersive X-ray and X-ray diffraction analysis. At one-day, antibacterial properties of material surfaces and leachates were tested against Enterococcus faecalis and Staphylococcus epidermidis biofilms; leachate cytotoxicity and cytokine release were assessed with the 3-(4,5 dimethylthiazolyl-2-yl)-2,5-diphenyl tetrazolium bromide assay and an enzyme-linked immunosorbent assay. Regression models were fitted for all dependent variables. Results: Compared to TZ-base, the BG-containing materials had overall increased water sorption, water uptake and porosity; decreased solubility in DMEM-sup; lower calcium release; and generally similar pH, antibacterial activity, cytotoxicity and cytokine release. All experimental cements were more cytotoxic than Biodentine, but the latter caused higher cytokine release. Significance: The BG-incorporation whether added or replacing the cement decreased the solubility in clinically relevant media and did not deteriorate the antimicrobial and biological properties of the materials. © 2025 The Authors
Ricci, Rafaela; Pereira, Bharbara Moura; Alvarado, Julissa Denisse Arguello; Sales-Junior, Romulo Oliveira; Machado, Nathália Evelyn Silva; Santos, Doany Cevada; Pederro, Felipe Haddad Martim; Magnani, Marciane; dos Santos Lima, Marcos; Ervolino, Edilson; Cintra, Luciano Tavares Ângelo; Kishen, Anil; Gomes-Filho, João Eduardo
Impact of Wine Polyphenols on the Inflammatory Profile of Induced Apical Periodontitis in Rats Journal Article
In: Journal of Endodontics, vol. 51, no. 5, pp. 594 – 601, 2025, ISSN: 00992399, (Cited by: 2).
@article{Ricci2025594,
title = {Impact of Wine Polyphenols on the Inflammatory Profile of Induced Apical Periodontitis in Rats},
author = {Rafaela Ricci and Bharbara Moura Pereira and Julissa Denisse Arguello Alvarado and Romulo Oliveira Sales-Junior and Nathália Evelyn Silva Machado and Doany Cevada Santos and Felipe Haddad Martim Pederro and Marciane Magnani and Marcos dos Santos Lima and Edilson Ervolino and Luciano Tavares Ângelo Cintra and Anil Kishen and João Eduardo Gomes-Filho},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85218076273&doi=10.1016%2fj.joen.2025.01.023&partnerID=40&md5=e58e54ece6102f1bdc20937880ac21b0},
doi = {10.1016/j.joen.2025.01.023},
issn = {00992399},
year = {2025},
date = {2025-01-01},
journal = {Journal of Endodontics},
volume = {51},
number = {5},
pages = {594 – 601},
publisher = {Elsevier Inc.},
abstract = {Introduction: This study evaluated the impact of dealcoholized red wine polyphenols on the inflammation and lesion volume associated with apical periodontitis (AP) in rats. Methods: Thirty-two Wistar rats receiving AP induction were arranged as follows: Control Group, Dealcoholized Red Wine Group (DRW), Red Wine Group, and Alcohol Group (ALC). Solutions were administered daily in a volume of 4.28 mL/kg via gavage for 45 days. Mandibles and maxillae were removed for histologic, immunohistochemical (IL-1β, IL-10, tumor necrosis factor-alpha, receptor activator of nuclear factor κB ligand, osteoprotegerin [OPG], and tartrate-resistant acid phosphatase), and micro-computed tomography analyses of the AP site. A statistical analysis was performed with a significance level of 5%. Results: Inflammation and TRAP-positive cell count were similar for DRW and Red Wine Group, but lower when compared to Control Group and ALC (P <.001). The immunohistochemical expression of OPG was higher for DRW than for ALC (P <.05). A larger lesion volume was observed in ALC compared to other groups (P <.001). Conclusions: Prophylactic administration of dealcoholized red wine significantly reduced inflammation, decreased the number of TRAP-positive cells, enhanced OPG expression, and reduced lesion volume compared to water and alcohol solutions. © 2025 American Association of Endodontists},
note = {Cited by: 2},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Introduction: This study evaluated the impact of dealcoholized red wine polyphenols on the inflammation and lesion volume associated with apical periodontitis (AP) in rats. Methods: Thirty-two Wistar rats receiving AP induction were arranged as follows: Control Group, Dealcoholized Red Wine Group (DRW), Red Wine Group, and Alcohol Group (ALC). Solutions were administered daily in a volume of 4.28 mL/kg via gavage for 45 days. Mandibles and maxillae were removed for histologic, immunohistochemical (IL-1β, IL-10, tumor necrosis factor-alpha, receptor activator of nuclear factor κB ligand, osteoprotegerin [OPG], and tartrate-resistant acid phosphatase), and micro-computed tomography analyses of the AP site. A statistical analysis was performed with a significance level of 5%. Results: Inflammation and TRAP-positive cell count were similar for DRW and Red Wine Group, but lower when compared to Control Group and ALC (P <.001). The immunohistochemical expression of OPG was higher for DRW than for ALC (P <.05). A larger lesion volume was observed in ALC compared to other groups (P <.001). Conclusions: Prophylactic administration of dealcoholized red wine significantly reduced inflammation, decreased the number of TRAP-positive cells, enhanced OPG expression, and reduced lesion volume compared to water and alcohol solutions. © 2025 American Association of Endodontists
Zanjir, Maryam; Cardoso, Elaine; Harman, Nicola L.; Khansari, Ava; Jafarzadeh, Hamid; Malkhassian, Gevik; Sabeti, Mike; Aminoshariae, Anita; Kishen, Anil; Sale, Joanna E. M.; Shah, Prakesh S.; Azarpazhooh, Amir
Development of a Core Outcome Set in Endodontics (COS-ENDO): Part 4 – COS-ENDO for Studies of Vital Pulp Therapy in Permanent Teeth Journal Article
In: Journal of Endodontics, vol. 51, no. 4, pp. 442 – 456, 2025, ISSN: 00992399, (Cited by: 5).
@article{Zanjir2025442,
title = {Development of a Core Outcome Set in Endodontics (COS-ENDO): Part 4 – COS-ENDO for Studies of Vital Pulp Therapy in Permanent Teeth},
author = {Maryam Zanjir and Elaine Cardoso and Nicola L. Harman and Ava Khansari and Hamid Jafarzadeh and Gevik Malkhassian and Mike Sabeti and Anita Aminoshariae and Anil Kishen and Joanna E. M. Sale and Prakesh S. Shah and Amir Azarpazhooh},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-105003479780&doi=10.1016%2fj.joen.2025.01.009&partnerID=40&md5=db44d229954ce2480b11c890b6aa5ece},
doi = {10.1016/j.joen.2025.01.009},
issn = {00992399},
year = {2025},
date = {2025-01-01},
journal = {Journal of Endodontics},
volume = {51},
number = {4},
pages = {442 – 456},
publisher = {Elsevier Inc.},
abstract = {Introduction: Methodological heterogeneity and reporting bias complicate interpreting endodontic outcomes. Supported by the American Association of Endodontists and its Foundation, this study developed the Core Outcome Set for Endodontics (COS-ENDO) to standardize reporting for studies of Nonsurgical Root Canal Treatment/Retreatment, Surgical Endodontics, Vital Pulp Therapy, Apexification, and Regenerative Endodontics. This article, Part 4 of a 5-part series, focuses on COS-ENDO for Vital Pulp Therapy, while other parts address the remaining treatments. Methods: Outcomes were identified through a scoping review and a web-based survey of American Association of Endodontists practicing members. In a 2-round Delphi survey, clinicians, patients, and researchers rated outcomes on a 9-point scale. Ratings were assessed against consensus criteria, and a virtual meeting finalized COS-ENDO. Results: Of the 97 invited participants to Round 1, 77 registered, and 73 (24 Academicians/Researchers, 26 Clinicians, and 23 Patients or their parents/caregivers) assessed 46 outcomes, with 17 excluded. The remaining outcomes—4 achieving consensus for inclusion and 25 lacking consensus—advanced to Round 2 for re-rating by 70 participants. This resulted in 10 outcomes achieving consensus for inclusion, and 19 lacking consensus. In the final meeting, 16 participants finalized the COS-ENDO for Vital Pulp Therapy studies, including Tooth survival, Pain, Signs of infection, Radiographic evidence of maintained periradicular health, Continued root development, Success, Functional tooth, Need for further intervention, and Adverse events/Complications. Conclusion: COS-ENDO is an important step in standardizing outcome reporting in endodontics, ensuring consistent reporting and enhancing research utility. Further efforts are needed to establish optimal measurement methods. © 2025 The Authors},
note = {Cited by: 5},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Introduction: Methodological heterogeneity and reporting bias complicate interpreting endodontic outcomes. Supported by the American Association of Endodontists and its Foundation, this study developed the Core Outcome Set for Endodontics (COS-ENDO) to standardize reporting for studies of Nonsurgical Root Canal Treatment/Retreatment, Surgical Endodontics, Vital Pulp Therapy, Apexification, and Regenerative Endodontics. This article, Part 4 of a 5-part series, focuses on COS-ENDO for Vital Pulp Therapy, while other parts address the remaining treatments. Methods: Outcomes were identified through a scoping review and a web-based survey of American Association of Endodontists practicing members. In a 2-round Delphi survey, clinicians, patients, and researchers rated outcomes on a 9-point scale. Ratings were assessed against consensus criteria, and a virtual meeting finalized COS-ENDO. Results: Of the 97 invited participants to Round 1, 77 registered, and 73 (24 Academicians/Researchers, 26 Clinicians, and 23 Patients or their parents/caregivers) assessed 46 outcomes, with 17 excluded. The remaining outcomes—4 achieving consensus for inclusion and 25 lacking consensus—advanced to Round 2 for re-rating by 70 participants. This resulted in 10 outcomes achieving consensus for inclusion, and 19 lacking consensus. In the final meeting, 16 participants finalized the COS-ENDO for Vital Pulp Therapy studies, including Tooth survival, Pain, Signs of infection, Radiographic evidence of maintained periradicular health, Continued root development, Success, Functional tooth, Need for further intervention, and Adverse events/Complications. Conclusion: COS-ENDO is an important step in standardizing outcome reporting in endodontics, ensuring consistent reporting and enhancing research utility. Further efforts are needed to establish optimal measurement methods. © 2025 The Authors
Aminoshariae, Anita; Azarpazhooh, Amir; Fouad, Ashraf F.; Glickman, Gerald N.; He, Jianing; Kim, Sahng G.; Kishen, Anil; Letra, Ariadne M.; Levin, Linda; Setzer, Frank C.; Tay, Franklin R.; Hargreaves, Kenneth M.
Insights into the May 2024 Issue of the Journal of Endodontics Journal Article
In: Journal of Endodontics, vol. 50, no. 5, pp. 549 – 551, 2024, ISSN: 00992399, (Cited by: 0).
@article{Aminoshariae2024549,
title = {Insights into the May 2024 Issue of the Journal of Endodontics},
author = {Anita Aminoshariae and Amir Azarpazhooh and Ashraf F. Fouad and Gerald N. Glickman and Jianing He and Sahng G. Kim and Anil Kishen and Ariadne M. Letra and Linda Levin and Frank C. Setzer and Franklin R. Tay and Kenneth M. Hargreaves},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85190523387&doi=10.1016%2fj.joen.2024.04.001&partnerID=40&md5=4a4d75b92af5ff1a9141bfeb97855b4b},
doi = {10.1016/j.joen.2024.04.001},
issn = {00992399},
year = {2024},
date = {2024-01-01},
journal = {Journal of Endodontics},
volume = {50},
number = {5},
pages = {549 – 551},
publisher = {Elsevier Inc.},
note = {Cited by: 0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Moriya, Paulo Takeji; Carvalho, Kleber Kildare Teodoro; Kishen, Anil; Souza, Erick M.; Versiani, Marco A.
In: Journal of Endodontics, vol. 50, no. 8, pp. 1151 – 1158, 2024, ISSN: 00992399, (Cited by: 1).
@article{Moriya20241151,
title = {Dynamic Photoelastic Analysis of Stress Distribution in Simulated Canals Using Rotary Instruments with Varied Tip and Taper Sizes: A Quasi-3D Approach},
author = {Paulo Takeji Moriya and Kleber Kildare Teodoro Carvalho and Anil Kishen and Erick M. Souza and Marco A. Versiani},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85195866928&doi=10.1016%2fj.joen.2024.05.008&partnerID=40&md5=d80cc216f78560c36bbc5fe842945cd8},
doi = {10.1016/j.joen.2024.05.008},
issn = {00992399},
year = {2024},
date = {2024-01-01},
journal = {Journal of Endodontics},
volume = {50},
number = {8},
pages = {1151 – 1158},
publisher = {Elsevier Inc.},
abstract = {Introduction: To compare the stress produced on the walls of simulated canals by rotary instruments with varied tip and taper sizes. Methods: Ninety isotropic transparent blocks, each containing a 60-degree curved canal, were distributed into 18 groups (n = 5) based on the instrument tip (sizes 10, 15, 20, 25, 30, and 35) and taper (sizes 0.02, 0.04, and 0.06). The blocks were fixed in a circular polariscope setup for dark field analysis. A digital camera was employed to capture the real-time birefringence patterns generated by each instrument. Digital image frames, corresponding to the instrument reaching the end of each canal third, were extracted and evaluated by 2 independent observers for the stress generation on canal walls. The data analysis employed a semi-quantitative scale ranging from 0 to 5. Cohen's Kappa coefficient test was used to determine the inter-observer agreement while the results were compared using Kruskal–Wallis test followed by an all-pairwise posthoc procedure (α = 5%). Results: Inter-observer agreement was 0.95. A significant influence of the tip size on stress was observed across the coronal (P = .011), middle (P = .006), and apical (P = .026) thirds. In contrast, taper size did not affect the stress induced at the coronal (P = .509), middle (P = .958), or apical (P = .493) thirds. The variations in tip and taper sizes did not result in a significant stress differences among the thirds (P = .181). Conclusions: The stress significantly increased across all canal thirds with larger tip sizes of rotary instruments, whereas the taper sizes did not influence the stress when compared to the canal thirds. © 2024 American Association of Endodontists},
note = {Cited by: 1},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Introduction: To compare the stress produced on the walls of simulated canals by rotary instruments with varied tip and taper sizes. Methods: Ninety isotropic transparent blocks, each containing a 60-degree curved canal, were distributed into 18 groups (n = 5) based on the instrument tip (sizes 10, 15, 20, 25, 30, and 35) and taper (sizes 0.02, 0.04, and 0.06). The blocks were fixed in a circular polariscope setup for dark field analysis. A digital camera was employed to capture the real-time birefringence patterns generated by each instrument. Digital image frames, corresponding to the instrument reaching the end of each canal third, were extracted and evaluated by 2 independent observers for the stress generation on canal walls. The data analysis employed a semi-quantitative scale ranging from 0 to 5. Cohen's Kappa coefficient test was used to determine the inter-observer agreement while the results were compared using Kruskal–Wallis test followed by an all-pairwise posthoc procedure (α = 5%). Results: Inter-observer agreement was 0.95. A significant influence of the tip size on stress was observed across the coronal (P = .011), middle (P = .006), and apical (P = .026) thirds. In contrast, taper size did not affect the stress induced at the coronal (P = .509), middle (P = .958), or apical (P = .493) thirds. The variations in tip and taper sizes did not result in a significant stress differences among the thirds (P = .181). Conclusions: The stress significantly increased across all canal thirds with larger tip sizes of rotary instruments, whereas the taper sizes did not influence the stress when compared to the canal thirds. © 2024 American Association of Endodontists
Rajeshwari, Hadagalu Revana Siddappa; Bishop, Emily; Ali, Aiman; Kishen, Anil
In: Bioactive Materials, vol. 41, pp. 400 – 412, 2024, ISSN: 2452199X, (Cited by: 0; All Open Access, Gold Open Access, Green Open Access).
@article{Rajeshwari2024400,
title = {Deciphering 3D periodontal fibroblast-macrophage crosstalk in bioactive nanoparticle-guided immunomodulation for treating traumatic dental avulsion},
author = {Hadagalu Revana Siddappa Rajeshwari and Emily Bishop and Aiman Ali and Anil Kishen},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85199889931&doi=10.1016%2fj.bioactmat.2024.07.017&partnerID=40&md5=42cdc46d0d13ce788eaf6d625b62c83e},
doi = {10.1016/j.bioactmat.2024.07.017},
issn = {2452199X},
year = {2024},
date = {2024-01-01},
journal = {Bioactive Materials},
volume = {41},
pages = {400 – 412},
publisher = {KeAi Communications Co.},
abstract = {Prolonged extra-oral period in dental avulsion is often associated with loss of viability of Periodontal fibroblasts (PDLF) and increased risk of ankylosis. Root surface treatment with bioactive agents to reduce the risk of ankylosis can be a potential strategy. The objective of the study was to investigate the impact of an engineered chitosan nanoparticles (CSNP), photosensitizer Rose Bengal (RB) functionalized CSNP (CSRB) and sustained dexamethasone (CSDEX) releasing CSNP for application in management of delayed replantation of avulsed teeth. The 3D PDLF-macrophage (Mϕ) collagen model was developed and exposed to LPS, MCSF, RANKL with and without CSDEX/CSNP. Immunofluorescence and cytokine analysis was done at 2 and 7 days to assess cellular interactions. Maxillary right incisors in male Wistar rats were extracted, exposed to extraoral dry or LPS for 1 h and treated with or without CSDEX/CSRB for 1 min before replantation. Rats were euthanized after 21 days for micro-CT, TRAP, and immunofluorescence analysis. CSDEX/CSNP treatment in 3D model significantly reduced CD80, NFATc1, STAT6 and increased CD206 and periostin expression (p < 0.05). TNFα, MMP9 was downregulated and IL10, TGFβ1, MMP2 upregulated with CSDEX/CSNP (p < 0.05). CSDEX/CSRB in animal study significantly reduced resorption, ankylosis, TRAP activity and osteocalcin expression and increased periostin (p<0.05). CSDEX demonstrated higher anti-inflammatory activity by downregulating TNFα, while CSNP upregulated TGFβ1, periostin, and downregulated MMP9. The combination of matrix stabilization with CSRB with periostin upregulation and sustained releasing CSDEX showed potential for hampering root resorption and ankylosis in dental avulsion. © 2024 The Authors},
note = {Cited by: 0; All Open Access, Gold Open Access, Green Open Access},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Prolonged extra-oral period in dental avulsion is often associated with loss of viability of Periodontal fibroblasts (PDLF) and increased risk of ankylosis. Root surface treatment with bioactive agents to reduce the risk of ankylosis can be a potential strategy. The objective of the study was to investigate the impact of an engineered chitosan nanoparticles (CSNP), photosensitizer Rose Bengal (RB) functionalized CSNP (CSRB) and sustained dexamethasone (CSDEX) releasing CSNP for application in management of delayed replantation of avulsed teeth. The 3D PDLF-macrophage (Mϕ) collagen model was developed and exposed to LPS, MCSF, RANKL with and without CSDEX/CSNP. Immunofluorescence and cytokine analysis was done at 2 and 7 days to assess cellular interactions. Maxillary right incisors in male Wistar rats were extracted, exposed to extraoral dry or LPS for 1 h and treated with or without CSDEX/CSRB for 1 min before replantation. Rats were euthanized after 21 days for micro-CT, TRAP, and immunofluorescence analysis. CSDEX/CSNP treatment in 3D model significantly reduced CD80, NFATc1, STAT6 and increased CD206 and periostin expression (p < 0.05). TNFα, MMP9 was downregulated and IL10, TGFβ1, MMP2 upregulated with CSDEX/CSNP (p < 0.05). CSDEX/CSRB in animal study significantly reduced resorption, ankylosis, TRAP activity and osteocalcin expression and increased periostin (p<0.05). CSDEX demonstrated higher anti-inflammatory activity by downregulating TNFα, while CSNP upregulated TGFβ1, periostin, and downregulated MMP9. The combination of matrix stabilization with CSRB with periostin upregulation and sustained releasing CSDEX showed potential for hampering root resorption and ankylosis in dental avulsion. © 2024 The Authors
Aminoshariae, Anita; Azarpazhooh, Amir; Fouad, Ashraf F.; Glickman, Gerald N.; He, Jianing; Kim, Sahng G.; Kishen, Anil; Letra, Ariadne M.; Levin, Linda; Setzer, Frank C.; Tay, Franklin R.; Hargreaves, Kenneth M.
Insights Into the December 2024 Issue of the Journal of Endodontics Journal Article
In: Journal of Endodontics, vol. 50, no. 12, pp. 1695 – 1697, 2024, ISSN: 00992399, (Cited by: 0).
@article{Aminoshariae20241695,
title = {Insights Into the December 2024 Issue of the Journal of Endodontics},
author = {Anita Aminoshariae and Amir Azarpazhooh and Ashraf F. Fouad and Gerald N. Glickman and Jianing He and Sahng G. Kim and Anil Kishen and Ariadne M. Letra and Linda Levin and Frank C. Setzer and Franklin R. Tay and Kenneth M. Hargreaves},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85212578515&doi=10.1016%2fj.joen.2024.12.004&partnerID=40&md5=4143d54129ad54ef42eae250d92a812a},
doi = {10.1016/j.joen.2024.12.004},
issn = {00992399},
year = {2024},
date = {2024-01-01},
journal = {Journal of Endodontics},
volume = {50},
number = {12},
pages = {1695 – 1697},
publisher = {Elsevier Inc.},
note = {Cited by: 0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Aminoshariae, Anita; Azarpazhooh, Amir; Fouad, Ashraf F.; Glickman, Gerald N.; He, Jianing; Kim, Sahng G.; Kishen, Anil; Letra, Ariadne M.; Levin, Linda; Setzer, Frank C.; Tay, Franklin R.; Hargreaves, Kenneth M.
Insights into the January 2024 Issue of the Journal of Endodontics Journal Article
In: Journal of Endodontics, vol. 50, no. 1, pp. 1 – 3, 2024, ISSN: 00992399, (Cited by: 0).
@article{Aminoshariae20241,
title = {Insights into the January 2024 Issue of the Journal of Endodontics},
author = {Anita Aminoshariae and Amir Azarpazhooh and Ashraf F. Fouad and Gerald N. Glickman and Jianing He and Sahng G. Kim and Anil Kishen and Ariadne M. Letra and Linda Levin and Frank C. Setzer and Franklin R. Tay and Kenneth M. Hargreaves},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85178570457&doi=10.1016%2fj.joen.2023.11.012&partnerID=40&md5=0dff919d381faed5b6ddc63fdb2f7904},
doi = {10.1016/j.joen.2023.11.012},
issn = {00992399},
year = {2024},
date = {2024-01-01},
journal = {Journal of Endodontics},
volume = {50},
number = {1},
pages = {1 – 3},
publisher = {Elsevier Inc.},
note = {Cited by: 0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Sharma, Shivam; Kishen, Anil
Bioarchitectural Design of Bioactive Biopolymers: Structure–Function Paradigm for Diabetic Wound Healing Journal Article
In: Biomimetics, vol. 9, no. 5, 2024, ISSN: 23137673, (Cited by: 4; All Open Access, Gold Open Access, Green Open Access).
@article{Sharma2024,
title = {Bioarchitectural Design of Bioactive Biopolymers: Structure–Function Paradigm for Diabetic Wound Healing},
author = {Shivam Sharma and Anil Kishen},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85194093659&doi=10.3390%2fbiomimetics9050275&partnerID=40&md5=04a808cf8e43e46f26c6383dbbd1f0b9},
doi = {10.3390/biomimetics9050275},
issn = {23137673},
year = {2024},
date = {2024-01-01},
journal = {Biomimetics},
volume = {9},
number = {5},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
abstract = {Chronic wounds such as diabetic ulcers are a major complication in diabetes caused by hyperglycemia, prolonged inflammation, high oxidative stress, and bacterial bioburden. Bioactive biopolymers have been found to have a biological response in wound tissue microenvironments and are used for developing advanced tissue engineering strategies to enhance wound healing. These biopolymers possess innate bioactivity and are biodegradable, with favourable mechanical properties. However, their bioactivity is highly dependent on their structural properties, which need to be carefully considered while developing wound healing strategies. Biopolymers such as alginate, chitosan, hyaluronic acid, and collagen have previously been used in wound healing solutions but the modulation of structural/physico-chemical properties for differential bioactivity have not been the prime focus. Factors such as molecular weight, degree of polymerization, amino acid sequences, and hierarchical structures can have a spectrum of immunomodulatory, anti-bacterial, and anti-oxidant properties that could determine the fate of the wound. The current narrative review addresses the structure–function relationship in bioactive biopolymers for promoting healing in chronic wounds with emphasis on diabetic ulcers. This review highlights the need for characterization of the biopolymers under research while designing biomaterials to maximize the inherent bioactive potency for better tissue regeneration outcomes, especially in the context of diabetic ulcers. © 2024 by the authors.},
note = {Cited by: 4; All Open Access, Gold Open Access, Green Open Access},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Chronic wounds such as diabetic ulcers are a major complication in diabetes caused by hyperglycemia, prolonged inflammation, high oxidative stress, and bacterial bioburden. Bioactive biopolymers have been found to have a biological response in wound tissue microenvironments and are used for developing advanced tissue engineering strategies to enhance wound healing. These biopolymers possess innate bioactivity and are biodegradable, with favourable mechanical properties. However, their bioactivity is highly dependent on their structural properties, which need to be carefully considered while developing wound healing strategies. Biopolymers such as alginate, chitosan, hyaluronic acid, and collagen have previously been used in wound healing solutions but the modulation of structural/physico-chemical properties for differential bioactivity have not been the prime focus. Factors such as molecular weight, degree of polymerization, amino acid sequences, and hierarchical structures can have a spectrum of immunomodulatory, anti-bacterial, and anti-oxidant properties that could determine the fate of the wound. The current narrative review addresses the structure–function relationship in bioactive biopolymers for promoting healing in chronic wounds with emphasis on diabetic ulcers. This review highlights the need for characterization of the biopolymers under research while designing biomaterials to maximize the inherent bioactive potency for better tissue regeneration outcomes, especially in the context of diabetic ulcers. © 2024 by the authors.
Aminoshariae, Anita; Azarpazhooh, Amir; Fouad, Ashraf F.; Glickman, Gerald N.; He, Jianing; Kim, Sahng G.; Kishen, Anil; Letra, Ariadne M.; Levin, Linda; Setzer, Frank C.; Tay, Franklin R.; Hargreaves, Kenneth M.
Insights Into the Oct 2024 Issue of the Journal of Endodontics Journal Article
In: Journal of Endodontics, vol. 50, no. 10, pp. 1367 – 1369, 2024, ISSN: 00992399, (Cited by: 0).
@article{Aminoshariae20241367,
title = {Insights Into the Oct 2024 Issue of the Journal of Endodontics},
author = {Anita Aminoshariae and Amir Azarpazhooh and Ashraf F. Fouad and Gerald N. Glickman and Jianing He and Sahng G. Kim and Anil Kishen and Ariadne M. Letra and Linda Levin and Frank C. Setzer and Franklin R. Tay and Kenneth M. Hargreaves},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85205673595&doi=10.1016%2fj.joen.2024.09.003&partnerID=40&md5=a925876aafe9af6bbb7a924a3f72d964},
doi = {10.1016/j.joen.2024.09.003},
issn = {00992399},
year = {2024},
date = {2024-01-01},
journal = {Journal of Endodontics},
volume = {50},
number = {10},
pages = {1367 – 1369},
publisher = {Elsevier Inc.},
note = {Cited by: 0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Aminoshariae, Anita; Azarpazhooh, Amir; Fouad, Ashraf F.; Glickman, Gerald N.; He, Jianing; Kim, Sahng G.; Kishen, Anil; Letra, Ariadne M.; Levin, Linda; Setzer, Frank C.; Tay, Franklin R.; Hargreaves, Kenneth M.
Insights into the June 2024 Issue of the JOE Journal Article
In: Journal of Endodontics, vol. 50, no. 6, pp. 721 – 723, 2024, ISSN: 00992399, (Cited by: 0).
@article{Aminoshariae2024721,
title = {Insights into the June 2024 Issue of the JOE},
author = {Anita Aminoshariae and Amir Azarpazhooh and Ashraf F. Fouad and Gerald N. Glickman and Jianing He and Sahng G. Kim and Anil Kishen and Ariadne M. Letra and Linda Levin and Frank C. Setzer and Franklin R. Tay and Kenneth M. Hargreaves},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85193034702&doi=10.1016%2fj.joen.2024.04.013&partnerID=40&md5=3132e6a5abe8a2f6aa672c09b3c4ab84},
doi = {10.1016/j.joen.2024.04.013},
issn = {00992399},
year = {2024},
date = {2024-01-01},
journal = {Journal of Endodontics},
volume = {50},
number = {6},
pages = {721 – 723},
publisher = {Elsevier Inc.},
note = {Cited by: 0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Aminoshariae, Anita; Azarpazhooh, Amir; Fouad, Ashraf F.; Glickman, Gerald N.; He, Jianing; Kim, Sahng G.; Kishen, Anil; Letra, Ariadne M.; Levin, Linda; Setzer, Frank C.; Tay, Franklin R.; Hargreaves, Kenneth M.
Insights into the March 2024 Issue of the JOE Journal Article
In: Journal of Endodontics, vol. 50, no. 3, pp. 289 – 291, 2024, ISSN: 00992399, (Cited by: 0).
@article{Aminoshariae2024289,
title = {Insights into the March 2024 Issue of the JOE},
author = {Anita Aminoshariae and Amir Azarpazhooh and Ashraf F. Fouad and Gerald N. Glickman and Jianing He and Sahng G. Kim and Anil Kishen and Ariadne M. Letra and Linda Levin and Frank C. Setzer and Franklin R. Tay and Kenneth M. Hargreaves},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85185576014&doi=10.1016%2fj.joen.2024.02.001&partnerID=40&md5=f15f4fd16a4b10d4ebe7ab49dab63369},
doi = {10.1016/j.joen.2024.02.001},
issn = {00992399},
year = {2024},
date = {2024-01-01},
journal = {Journal of Endodontics},
volume = {50},
number = {3},
pages = {289 – 291},
publisher = {Elsevier Inc.},
note = {Cited by: 0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
PradeepKumar, Angambakkam Rajasekaran; JothiLatha, Sundaramurthy; Durvasulu, Archana; Muralidhar, Lavanya; Alagu, Jeffery Vimalesh; Shereen, Judith; Kishen, Anil
Impact of Type 2 Diabetes Mellitus on the Occurrence of Vertical Root Fracture: A Case Control Study Journal Article
In: Journal of Endodontics, vol. 50, no. 4, pp. 450 – 455.e1, 2024, ISSN: 00992399, (Cited by: 5; All Open Access, Hybrid Gold Open Access).
@article{PradeepKumar2024450,
title = {Impact of Type 2 Diabetes Mellitus on the Occurrence of Vertical Root Fracture: A Case Control Study},
author = {Angambakkam Rajasekaran PradeepKumar and Sundaramurthy JothiLatha and Archana Durvasulu and Lavanya Muralidhar and Jeffery Vimalesh Alagu and Judith Shereen and Anil Kishen},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85185610389&doi=10.1016%2fj.joen.2024.01.006&partnerID=40&md5=8fa67647f74cfe654fe4d4af5b90c1c8},
doi = {10.1016/j.joen.2024.01.006},
issn = {00992399},
year = {2024},
date = {2024-01-01},
journal = {Journal of Endodontics},
volume = {50},
number = {4},
pages = {450 – 455.e1},
publisher = {Elsevier Inc.},
abstract = {Introduction: The aim of this case–control study was to examine the relationship between type 2 diabetes mellitus (DM) and the occurrence of VRFs. The crack extension, dentin sclerosis, and chemical characteristics of root dentin in teeth with VRF from patients with/without DM were also compared. Methods: One hundred and thirty-two patients diagnosed with VRF in crowned root filled posterior teeth were selected. The study was conducted in 2 parts. In Part-1: The cases were matched with control teeth (1:1) for age (±5 years), sex, tooth type, apical extent of root filling, time period after root filling to a diagnosis of VRF, presence or absence of intracanal post and abutment status. The presence or absence of type 2 DM (HbA1c > 6.5) was recorded. In Part-2: The extracted teeth with VRF from the case control study were used to evaluate the extension of VRF, presence of sclerotic dentin and isthmus using a microscopic analysis; while the levels of pentosidine, collagen cross-linking ratio and mineral-collagen ratio were determined by Fourier-transform infrared spectroscopy. The distribution of DM between cases and controls was analyzed using Pearson Chi-Square test and Odds Ratio estimated. Chemical composition data was analyzed using Mann–Whitney test. The extent of sclerotic dentin was analyzed using Pearson Chi-Square test. Results: When compared to patients without DM, patients with DM had 2.67 (95% CI: 1.6–4.45) folds higher odds for occurrence of VRF. Pentosidine (P = .014), collagen cross-linking ratio(P = .047), mineral-collagen ratio (P = .009) and sclerotic dentin extent (P = .0009) were significantly higher in patients with DM and VRF. Conclusions: Type 2 DM was more often associated with VRFs in root canal treated teeth with crowns. Root dentin from patients with type 2 DM and VRF had higher levels of pentosidine, collagen cross-linking ratio, mineral to collagen ratio and sclerotic dentin. © 2024 The Authors},
note = {Cited by: 5; All Open Access, Hybrid Gold Open Access},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Introduction: The aim of this case–control study was to examine the relationship between type 2 diabetes mellitus (DM) and the occurrence of VRFs. The crack extension, dentin sclerosis, and chemical characteristics of root dentin in teeth with VRF from patients with/without DM were also compared. Methods: One hundred and thirty-two patients diagnosed with VRF in crowned root filled posterior teeth were selected. The study was conducted in 2 parts. In Part-1: The cases were matched with control teeth (1:1) for age (±5 years), sex, tooth type, apical extent of root filling, time period after root filling to a diagnosis of VRF, presence or absence of intracanal post and abutment status. The presence or absence of type 2 DM (HbA1c > 6.5) was recorded. In Part-2: The extracted teeth with VRF from the case control study were used to evaluate the extension of VRF, presence of sclerotic dentin and isthmus using a microscopic analysis; while the levels of pentosidine, collagen cross-linking ratio and mineral-collagen ratio were determined by Fourier-transform infrared spectroscopy. The distribution of DM between cases and controls was analyzed using Pearson Chi-Square test and Odds Ratio estimated. Chemical composition data was analyzed using Mann–Whitney test. The extent of sclerotic dentin was analyzed using Pearson Chi-Square test. Results: When compared to patients without DM, patients with DM had 2.67 (95% CI: 1.6–4.45) folds higher odds for occurrence of VRF. Pentosidine (P = .014), collagen cross-linking ratio(P = .047), mineral-collagen ratio (P = .009) and sclerotic dentin extent (P = .0009) were significantly higher in patients with DM and VRF. Conclusions: Type 2 DM was more often associated with VRFs in root canal treated teeth with crowns. Root dentin from patients with type 2 DM and VRF had higher levels of pentosidine, collagen cross-linking ratio, mineral to collagen ratio and sclerotic dentin. © 2024 The Authors
Aminoshariae, Anita; Azarpazhooh, Amir; Fouad, Ashraf F.; Glickman, Gerald N.; He, Jianing; Kim, Sahng G.; Kishen, Anil; Letra, Ariadne M.; Levin, Linda; Setzer, Frank C.; Tay, Franklin R.; Hargreaves, Kenneth M.
Insights into the February 2024 Issue of the Journal of Endodontics Journal Article
In: Journal of Endodontics, vol. 50, no. 2, pp. 117 – 119, 2024, ISSN: 00992399, (Cited by: 0).
@article{Aminoshariae2024117,
title = {Insights into the February 2024 Issue of the Journal of Endodontics},
author = {Anita Aminoshariae and Amir Azarpazhooh and Ashraf F. Fouad and Gerald N. Glickman and Jianing He and Sahng G. Kim and Anil Kishen and Ariadne M. Letra and Linda Levin and Frank C. Setzer and Franklin R. Tay and Kenneth M. Hargreaves},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85183571746&doi=10.1016%2fj.joen.2024.01.004&partnerID=40&md5=a88e278b247a8efb93b6ea2dfe995625},
doi = {10.1016/j.joen.2024.01.004},
issn = {00992399},
year = {2024},
date = {2024-01-01},
journal = {Journal of Endodontics},
volume = {50},
number = {2},
pages = {117 – 119},
publisher = {Elsevier Inc.},
note = {Cited by: 0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Karthikeyan, Balasubramanian Saravana; Madhubala, Manavalan Madhana; Rajkumar, G.; Dhivya, V.; Kishen, Anil; Srinivasan, Narasimhan; Mahalaxmi, Sekar
In: International Journal of Biological Macromolecules, vol. 271, 2024, ISSN: 01418130, (Cited by: 8).
@article{SaravanaKarthikeyan2024,
title = {Physico-chemical and biological characterization of synthetic and eggshell derived nanohydroxyapatite/carboxymethyl chitosan composites for pulp-dentin tissue engineering},
author = {Balasubramanian Saravana Karthikeyan and Manavalan Madhana Madhubala and G. Rajkumar and V. Dhivya and Anil Kishen and Narasimhan Srinivasan and Sekar Mahalaxmi},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85194036754&doi=10.1016%2fj.ijbiomac.2024.132620&partnerID=40&md5=26ba76e1329ac7df53126f8e2a8e5e80},
doi = {10.1016/j.ijbiomac.2024.132620},
issn = {01418130},
year = {2024},
date = {2024-01-01},
journal = {International Journal of Biological Macromolecules},
volume = {271},
publisher = {Elsevier B.V.},
abstract = {Hybrid nanohydroxyapatite/carboxymethyl chitosan (nHAp-CMC) scaffolds have garnered significant attention in the field of regenerative engineering. The current study comparatively analyzed the physicochemical and biological properties of synthetic nanohydroxyapatite (SnHA)- and eggshell-sourced nanohydroxyapatite (EnHA)- based CMC biocomposites for pulp–dentin regeneration. EnHA and CMC were synthesized through a chemical process, whereas SnHA was commercially obtained. Composite scaffolds of SnHA-CMC and EnHA-CMC (1:5 w/w) were prepared using freeze-drying method. All biomaterials were characterized by FTIR, micro-Raman, XRD, HRSEM-EDX, and TEM analyses, and their in vitro bioactivity was assessed by immersing them in simulated body fluid for 21 days. The biological properties of the composite scaffolds were evaluated by assessing cytocompatibility using MTT assay and biomineralization potential by analyzing the odontogenic gene expressions (ALP, DSPP, DMP-1 and VEGF) in human dental pulp stem cells (DPSCs) using RT-qPCR method. Characterization studies revealed that EnHA displayed higher crystallinity and superior surface morphology compared to SnHA. The composite scaffolds showed a highly interconnected porous microstructure with pore sizes ranging between 60 and 220 μm, ideal for cell seeding. All tested materials, SnHA, EnHA, and their respective composites, displayed high cytocompatibility, increased ALP activity and degree of mineralization with significant upregulation of odontogenic-related genes on DPSCs (p < 0.05). Nevertheless, the odontogenic differentiation potential of EnHA-CMC on DPSCs was significantly higher when compared to SnHA-CMC. The findings from this study highlight the potential of EnHA-CMC as a promising candidate for pulp–dentin engineering. © 2024 Elsevier B.V.},
note = {Cited by: 8},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Hybrid nanohydroxyapatite/carboxymethyl chitosan (nHAp-CMC) scaffolds have garnered significant attention in the field of regenerative engineering. The current study comparatively analyzed the physicochemical and biological properties of synthetic nanohydroxyapatite (SnHA)- and eggshell-sourced nanohydroxyapatite (EnHA)- based CMC biocomposites for pulp–dentin regeneration. EnHA and CMC were synthesized through a chemical process, whereas SnHA was commercially obtained. Composite scaffolds of SnHA-CMC and EnHA-CMC (1:5 w/w) were prepared using freeze-drying method. All biomaterials were characterized by FTIR, micro-Raman, XRD, HRSEM-EDX, and TEM analyses, and their in vitro bioactivity was assessed by immersing them in simulated body fluid for 21 days. The biological properties of the composite scaffolds were evaluated by assessing cytocompatibility using MTT assay and biomineralization potential by analyzing the odontogenic gene expressions (ALP, DSPP, DMP-1 and VEGF) in human dental pulp stem cells (DPSCs) using RT-qPCR method. Characterization studies revealed that EnHA displayed higher crystallinity and superior surface morphology compared to SnHA. The composite scaffolds showed a highly interconnected porous microstructure with pore sizes ranging between 60 and 220 μm, ideal for cell seeding. All tested materials, SnHA, EnHA, and their respective composites, displayed high cytocompatibility, increased ALP activity and degree of mineralization with significant upregulation of odontogenic-related genes on DPSCs (p < 0.05). Nevertheless, the odontogenic differentiation potential of EnHA-CMC on DPSCs was significantly higher when compared to SnHA-CMC. The findings from this study highlight the potential of EnHA-CMC as a promising candidate for pulp–dentin engineering. © 2024 Elsevier B.V.
Aminoshariae, Anita; Azarpazhooh, Amir; Fouad, Ashraf F.; Glickman, Gerald N.; He, Jianing; Kim, Sahng G.; Kishen, Anil; Letra, Ariadne M.; Levin, Linda; Setzer, Frank C.; Tay, Franklin R.; Hargreaves, Kenneth M.
Insights into the September 2024 Issue of the Journal of Endodontics Journal Article
In: Journal of Endodontics, vol. 50, no. 9, pp. 1195 – 1197, 2024, ISSN: 00992399, (Cited by: 0).
@article{Aminoshariae20241195,
title = {Insights into the September 2024 Issue of the Journal of Endodontics},
author = {Anita Aminoshariae and Amir Azarpazhooh and Ashraf F. Fouad and Gerald N. Glickman and Jianing He and Sahng G. Kim and Anil Kishen and Ariadne M. Letra and Linda Levin and Frank C. Setzer and Franklin R. Tay and Kenneth M. Hargreaves},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85200969994&doi=10.1016%2fj.joen.2024.07.014&partnerID=40&md5=acf73722d9ddae44f4b07e84647afe6f},
doi = {10.1016/j.joen.2024.07.014},
issn = {00992399},
year = {2024},
date = {2024-01-01},
journal = {Journal of Endodontics},
volume = {50},
number = {9},
pages = {1195 – 1197},
publisher = {Elsevier Inc.},
note = {Cited by: 0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Siddappa, Rajeshwari Hadagalu Revana; Bishop, Emily; Ali, Aiman; Magalhaes, Marco; Kishen, Anil
Engineered Immunomodulatory Nanoparticles Inhibit Root Resorption and Ankylosis Journal Article
In: Journal of Endodontics, vol. 50, no. 11, pp. 1579 – 1592.e3, 2024, ISSN: 00992399, (Cited by: 1; All Open Access, Hybrid Gold Open Access).
@article{HadagaluRevanaSiddappa20241579,
title = {Engineered Immunomodulatory Nanoparticles Inhibit Root Resorption and Ankylosis},
author = {Rajeshwari Hadagalu Revana Siddappa and Emily Bishop and Aiman Ali and Marco Magalhaes and Anil Kishen},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85203532792&doi=10.1016%2fj.joen.2024.08.006&partnerID=40&md5=ff68a54805677b42428884d979f9a490},
doi = {10.1016/j.joen.2024.08.006},
issn = {00992399},
year = {2024},
date = {2024-01-01},
journal = {Journal of Endodontics},
volume = {50},
number = {11},
pages = {1579 – 1592.e3},
publisher = {Elsevier Inc.},
abstract = {Introduction: External root resorption following avulsion injury is a complex process wherein differentiation of macrophages (Mϕ) to multinucleated osteoclasts is temporally regulated by resident periodontal fibroblasts (PDLF). The current study aims to assess the effect of engineered bioactive chitosan nanoparticles (CSNP), sustained released dexamethasone conjugated CSNP (CS-DEX) and CSNP functionalized with photosensitizer Rose Bengal (CSRB) for application in root resorption using an in-vitro PDLF-Mϕ direct coculture model and in-vivo delayed reimplantation model. Methods: PDLF-Mϕ direct coculture system was exposed to lipopolysaccharide (LPS), macrophage colony stimulating factor, receptor activator of nuclear factor kappa β ligand with or without CSNP/CS-DEX for 7 days. Clastic differentiation was assessed by tartrate resistant acid phosphatase (TRAP) staining on day 7. On day 2 and 7, immunofluorescence analysis was conducted to assess the expression of Mϕ polarization markers (CD80, CD206), multinucleation markers (NFATc1, STAT6) in Mϕ and matricellular protein periostin in PDLF and cytokine profiling in cell culture supernatants. Delayed replantation model with extraoral air dry/LPS exposure for 1h followed by root surface treatment with CS-DEX/CSRB was used in Wistar rats. After 21 days, rats were euthanized for histologic and immunofluorescence analysis. Statistical analysis one-way ANOVA with Tukey's multiple comparisons was used to analyze the data (P < .05). Results: CS-DEX significantly reduced TRAP+ multinucleated cells and CSNP treatment showed no TRAP+ cells. Immunofluorescence analysis showed that CSNP/CS-DEX reduced CD80, NFATc1 and STAT6 expression and increased periostin as expressed by fluorescence intensity. CSNP/CS-DEX significantly reduced TNFα, MMP9 and increased IL10, TGFβ1. Osteoprotegerin was upregulated only by CSNP. Root surface treatment in delayed replantation model showed that CS-DEX and CSRB substantially reduced the degree of resorption and ankylosis. Further, CD80, CD206, and MMP2 expression in groups with root surface treatment with CS-DEX and CSRB was lower than airdry/LPS group and similar to healthy control and NFATc1, STAT6, and MMP9 expressions were lower than healthy control. Conclusion: The engineered nanosized immunomodulatory bioactive materials chitosan nanoparticles functionalized with photosensitizer and dexamethasone effectively reduced the clastic differentiation of Mϕ in in-vitro coculture and minimized the resorption and ankylosis in a delayed reimplantation model. These biomaterials have the potential to serve as root modification agents, promoting favorable healing outcomes in cases of dental avulsion. © 2024 The Authors},
note = {Cited by: 1; All Open Access, Hybrid Gold Open Access},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Introduction: External root resorption following avulsion injury is a complex process wherein differentiation of macrophages (Mϕ) to multinucleated osteoclasts is temporally regulated by resident periodontal fibroblasts (PDLF). The current study aims to assess the effect of engineered bioactive chitosan nanoparticles (CSNP), sustained released dexamethasone conjugated CSNP (CS-DEX) and CSNP functionalized with photosensitizer Rose Bengal (CSRB) for application in root resorption using an in-vitro PDLF-Mϕ direct coculture model and in-vivo delayed reimplantation model. Methods: PDLF-Mϕ direct coculture system was exposed to lipopolysaccharide (LPS), macrophage colony stimulating factor, receptor activator of nuclear factor kappa β ligand with or without CSNP/CS-DEX for 7 days. Clastic differentiation was assessed by tartrate resistant acid phosphatase (TRAP) staining on day 7. On day 2 and 7, immunofluorescence analysis was conducted to assess the expression of Mϕ polarization markers (CD80, CD206), multinucleation markers (NFATc1, STAT6) in Mϕ and matricellular protein periostin in PDLF and cytokine profiling in cell culture supernatants. Delayed replantation model with extraoral air dry/LPS exposure for 1h followed by root surface treatment with CS-DEX/CSRB was used in Wistar rats. After 21 days, rats were euthanized for histologic and immunofluorescence analysis. Statistical analysis one-way ANOVA with Tukey's multiple comparisons was used to analyze the data (P < .05). Results: CS-DEX significantly reduced TRAP+ multinucleated cells and CSNP treatment showed no TRAP+ cells. Immunofluorescence analysis showed that CSNP/CS-DEX reduced CD80, NFATc1 and STAT6 expression and increased periostin as expressed by fluorescence intensity. CSNP/CS-DEX significantly reduced TNFα, MMP9 and increased IL10, TGFβ1. Osteoprotegerin was upregulated only by CSNP. Root surface treatment in delayed replantation model showed that CS-DEX and CSRB substantially reduced the degree of resorption and ankylosis. Further, CD80, CD206, and MMP2 expression in groups with root surface treatment with CS-DEX and CSRB was lower than airdry/LPS group and similar to healthy control and NFATc1, STAT6, and MMP9 expressions were lower than healthy control. Conclusion: The engineered nanosized immunomodulatory bioactive materials chitosan nanoparticles functionalized with photosensitizer and dexamethasone effectively reduced the clastic differentiation of Mϕ in in-vitro coculture and minimized the resorption and ankylosis in a delayed reimplantation model. These biomaterials have the potential to serve as root modification agents, promoting favorable healing outcomes in cases of dental avulsion. © 2024 The Authors
Aminoshariae, Anita; Azarpazhooh, Amir; Fouad, Ashraf F.; Glickman, Gerald N.; He, Jianing; Kim, Sahng G.; Kishen, Anil; Letra, Ariadne M.; Levin, Linda; Setzer, Frank C.; Tay, Franklin R.; Hargreaves, Kenneth M.
Insights into the August 2024 Issue of the Journal of Endodontics Journal Article
In: Journal of Endodontics, vol. 50, no. 8, pp. 1033 – 1035, 2024, ISSN: 00992399, (Cited by: 0).
@article{Aminoshariae20241033,
title = {Insights into the August 2024 Issue of the Journal of Endodontics},
author = {Anita Aminoshariae and Amir Azarpazhooh and Ashraf F. Fouad and Gerald N. Glickman and Jianing He and Sahng G. Kim and Anil Kishen and Ariadne M. Letra and Linda Levin and Frank C. Setzer and Franklin R. Tay and Kenneth M. Hargreaves},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85199215773&doi=10.1016%2fj.joen.2024.06.014&partnerID=40&md5=7316e16ef23a35142dfae02fda0d358b},
doi = {10.1016/j.joen.2024.06.014},
issn = {00992399},
year = {2024},
date = {2024-01-01},
journal = {Journal of Endodontics},
volume = {50},
number = {8},
pages = {1033 – 1035},
publisher = {Elsevier Inc.},
note = {Cited by: 0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Aminoshariae, Anita; Azarpazhooh, Amir; Fouad, Ashraf F.; Glickman, Gerald N.; He, Jianing; Kim, Sahng G.; Kishen, Anil; Letra, Ariadne M.; Levin, Linda; Setzer, Frank C.; Tay, Franklin R.; Hargreaves, Kenneth M.
Insights into the July 2024 Issue of the Journal of Endodontics Journal Article
In: Journal of Endodontics, vol. 50, no. 7, pp. 875 – 877, 2024, ISSN: 00992399, (Cited by: 0).
@article{Aminoshariae2024875,
title = {Insights into the July 2024 Issue of the Journal of Endodontics},
author = {Anita Aminoshariae and Amir Azarpazhooh and Ashraf F. Fouad and Gerald N. Glickman and Jianing He and Sahng G. Kim and Anil Kishen and Ariadne M. Letra and Linda Levin and Frank C. Setzer and Franklin R. Tay and Kenneth M. Hargreaves},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85196042295&doi=10.1016%2fj.joen.2024.05.017&partnerID=40&md5=a5bdf8f899a765e1c5e6f3fa2c5df8e6},
doi = {10.1016/j.joen.2024.05.017},
issn = {00992399},
year = {2024},
date = {2024-01-01},
journal = {Journal of Endodontics},
volume = {50},
number = {7},
pages = {875 – 877},
publisher = {Elsevier Inc.},
note = {Cited by: 0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Aminoshariae, Anita; Azarpazhooh, Amir; Fouad, Ashraf F.; Glickman, Gerald N.; He, Jianing; Kim, Sahng G.; Kishen, Anil; Letra, Ariadne M.; Levin, Linda; Setzer, Frank C.; Tay, Franklin R.; Hargreaves, Kenneth M.
Insights into the April 2024 Issue of the Journal of Endodontics Journal Article
In: Journal of Endodontics, vol. 50, no. 4, pp. 403 – 405, 2024, ISSN: 00992399, (Cited by: 0).
@article{Aminoshariae2024403,
title = {Insights into the April 2024 Issue of the Journal of Endodontics},
author = {Anita Aminoshariae and Amir Azarpazhooh and Ashraf F. Fouad and Gerald N. Glickman and Jianing He and Sahng G. Kim and Anil Kishen and Ariadne M. Letra and Linda Levin and Frank C. Setzer and Franklin R. Tay and Kenneth M. Hargreaves},
url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85187988109&doi=10.1016%2fj.joen.2024.03.001&partnerID=40&md5=5b0f0b6c6e15f873953170d5b08ad2b7},
doi = {10.1016/j.joen.2024.03.001},
issn = {00992399},
year = {2024},
date = {2024-01-01},
journal = {Journal of Endodontics},
volume = {50},
number = {4},
pages = {403 – 405},
publisher = {Elsevier Inc.},
note = {Cited by: 0},
keywords = {},
pubstate = {published},
tppubtype = {article}
}