@article{Menon2023,

title = {Nociceptor–Macrophage Interactions in Apical Periodontitis: How Biomolecules Link Inflammation with Pain},

author = {Nandita Menon and Anil Kishen},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85168747326&doi=10.3390%2fbiom13081193&partnerID=40&md5=0803e2ba1e709eb873167dbf4e3d6ef1},

doi = {10.3390/biom13081193},

issn = {2218273X},

year  = {2023},

date = {2023-01-01},

journal = {Biomolecules},

volume = {13},

number = {8},

publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},

abstract = {Periradicular tissues have a rich supply of peripheral afferent neurons, also known as nociceptive neurons, originating from the trigeminal nerve. While their primary function is to relay pain signals to the brain, these are known to be involved in modulating innate and adaptive immunity by initiating neurogenic inflammation (NI). Studies have investigated neuroanatomy and measured the levels of biomolecules such as cytokines and neuropeptides in human saliva, gingival crevicular fluid, or blood/serum samples in apical periodontitis (AP) to validate the possible role of trigeminal nociceptors in inflammation and tissue regeneration. However, the contributions of nociceptors and the mechanisms involved in the neuro-immune interactions in AP are not fully understood. This narrative review addresses the complex biomolecular interactions of trigeminal nociceptors with macrophages, the effector cells of the innate immune system, in the clinical manifestations of AP. © 2023 by the authors.},

note = {Cited by: 5; All Open Access, Gold Open Access, Green Open Access},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Gomes20231,

title = {Irrigants and irrigation activation systems in Endodontics},

author = {Brenda P. F. A. Gomes and Emelly Aveiro and Anil Kishen},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85175592922&doi=10.1590%2f0103-6440202305577&partnerID=40&md5=131793be4fc39e1346f108b39581f886},

doi = {10.1590/0103-6440202305577},

issn = {01036440},

year  = {2023},

date = {2023-01-01},

journal = {Brazilian Dental Journal},

volume = {34},

number = {4},

pages = {1 – 33},

publisher = {Associacao Brasileira de Divulgacao Cientifica},

abstract = {Root canal infections are typically polymicrobial and involve strong bacterial interactions. The goal of endodontic treatment is to remove infected content from the root canal system to allow the healing of a pre-existing periapical lesion or to prevent infection of the periradicular tissues. Instrumentation alone is not capable of touching all of the root canal walls. Therefore, the irrigation process is an essential step in the endodontic treatment. However, due to the complex anatomy of the root canal system, this cleaning is very challenging. Although syringe and needle irrigation associated with the use of chemical substances is still the most used method, it does not guarantee optimal cleaning of the root canals. As a result, not only alternative irrigating substances but also numerous activation systems - which are technologies that aim to optimize the action of irrigating substances, both chemically and physically - have been developed. This work aimed to review the characteristics of both classic and current alternatives of irrigating substances and irrigation activation systems. © 2023, Associacao Brasileira de Divulgacao Cientifica. All rights reserved.},

note = {Cited by: 57; All Open Access, Green Open Access},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Aminoshariae20231411,

title = {Insights into the November 2023 Issue of the Journal of Endodontics},

author = {Anita Aminoshariae and Amir Azarpazhooh and Ashraf F. Fouad and Gerald N. Glickman and Jianing He and Sahng G. Kim and Anil Kishen and Ariadne M. Letra and Linda Levin and Frank C. Setzer and Franklin R. Tay and Kenneth M. Hargreaves},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85174185052&doi=10.1016%2fj.joen.2023.10.001&partnerID=40&md5=c117a51607a5b15b0f8d4bba7cd2f92e},

doi = {10.1016/j.joen.2023.10.001},

issn = {00992399},

year  = {2023},

date = {2023-01-01},

journal = {Journal of Endodontics},

volume = {49},

number = {11},

pages = {1411 – 1413},

publisher = {Elsevier Inc.},

note = {Cited by: 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Aminoshariae20231,

title = {Insights into the January 2023 Issue of the JOE},

author = {Anita Aminoshariae and Amir Azarpazhooh and Ashraf F. Fouad and Gerald N. Glickman and Jianing He and Sahng G. Kim and Anil Kishen and Ariadne M. Letra and Linda Levin and Frank C. Setzer and Franklin R. Tay and Kenneth M. Hargreaves},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85144221226&doi=10.1016%2fj.joen.2022.12.004&partnerID=40&md5=4c024a8c34cd314ae39287d53161a821},

doi = {10.1016/j.joen.2022.12.004},

issn = {00992399},

year  = {2023},

date = {2023-01-01},

journal = {Journal of Endodontics},

volume = {49},

number = {1},

pages = {1 – 3},

publisher = {Elsevier Inc.},

note = {Cited by: 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{MahdiSouzani2023,

title = {Impact of 3D collagen-based model and hydrostatic pressure on periodontal ligament fibroblast: A morpho-biochemical analysis},

author = {Armin Mahdi Souzani and Hadagalu Revana Siddappa Rajeshwari and P. Ravi Selvaganapathy and Anil Kishen},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85170221816&doi=10.1016%2fj.jmbbm.2023.106092&partnerID=40&md5=fd61ada21cdda169eacb108df21fa693},

doi = {10.1016/j.jmbbm.2023.106092},

issn = {17516161},

year  = {2023},

date = {2023-01-01},

journal = {Journal of the Mechanical Behavior of Biomedical Materials},

volume = {147},

publisher = {Elsevier Ltd},

abstract = {This study developed a customized hydrostatic pressure-based loading environment to investigate the effect of static hydrostatic pressure on the periodontal ligament fibroblasts (PDLf) in a three-dimensional (3D) collagen-based model. The cylindrical tissue constructs were comprised of PDL fibroblast cells seeded in type I collagen matrices and divided into three experimental groups: Control (no load), low-load (∼0.07 kPa), and high-load (∼60 kPa), all subjected to 24 h of experimental duration. Cells in the 3D construct were stained with fluorophore-conjugated antibodies for cytoskeletal protein F-actin and matricellular protein periostin. Cell culture supernatant was assessed for receptor activator of nuclear factor kappaB ligand (RANKL) and osteoprotegerin (OPG) expression. Transmission electron microscopy examined the contact between the cells and the collagen matrix. Ultrastructural changes in the 3D collagen matrix were also analyzed using scanning electron microscopy. Experiments were performed in triplicates, and data was analyzed using one-way ANOVA (p < 0.05). The 3D PDLf constructs from the low-load group demonstrated the highest levels of homogeneous cell distribution and higher expression of F-actin and periostin with enhanced interaction with the matrix. The collagen matrix in this group showed more closely packed fibers forming thicker bundles when compared to the control and the high-load 3D PDLf constructs. Nonuniform cell distribution with decreased expression of F-actin and periostin was observed in the control and high-load PDLf constructs. The high-load group showed the highest RANKL/OPG expression. This study demonstrated low-level hydrostatic pressure's role in regulating PDLf functions and extracellular matrix response, while excessive hydrostatic pressure may be detrimental to PDL fibroblast cell function. © 2023 Elsevier Ltd},

note = {Cited by: 1},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Aminoshariae2023459,

title = {Insights Into the May 2023 Issue of the JOE},

author = {Anita Aminoshariae and Amir Azarpazhooh and Ashraf F. Fouad and Gerald N. Glickman and Jianing He and Sahng G. Kim and Anil Kishen and Ariadne M. Letra and Linda Levin and Frank C. Setzer and Franklin R. Tay and Kenneth M. Hargreaves},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85153624837&doi=10.1016%2fj.joen.2023.03.014&partnerID=40&md5=ad1f77402776387230e5dd979b07e7f1},

doi = {10.1016/j.joen.2023.03.014},

issn = {00992399},

year  = {2023},

date = {2023-01-01},

journal = {Journal of Endodontics},

volume = {49},

number = {5},

pages = {459 – 461},

publisher = {Elsevier Inc.},

note = {Cited by: 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Aminoshariae2023937,

title = {Insights into the August 2023 Issue of the JOE},

author = {Anita Aminoshariae and Amir Azarpazhooh and Ashraf F. Fouad and Gerald N. Glickman and Jianing He and Sahng G. Kim and Anil Kishen and Ariadne M. Letra and Linda Levin and Frank C. Setzer and Franklin R. Tay and Kenneth M. Hargreaves},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85166442640&doi=10.1016%2fj.joen.2023.06.016&partnerID=40&md5=93749d8db3b053a934e2303d9527144f},

doi = {10.1016/j.joen.2023.06.016},

issn = {00992399},

year  = {2023},

date = {2023-01-01},

journal = {Journal of Endodontics},

volume = {49},

number = {8},

pages = {937 – 939},

publisher = {Elsevier Inc.},

note = {Cited by: 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Li2023,

title = {3D Organoids for Regenerative Endodontics},

author = {Fang-Chi Li and Anil Kishen},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85163840412&doi=10.3390%2fbiom13060900&partnerID=40&md5=4e4c3896011b9733139642631d448297},

doi = {10.3390/biom13060900},

issn = {2218273X},

year  = {2023},

date = {2023-01-01},

journal = {Biomolecules},

volume = {13},

number = {6},

publisher = {MDPI},

abstract = {Apical periodontitis is the inflammation and destruction of periradicular tissues, mediated by microbial factors originating from the infected pulp space. This bacteria-mediated inflammatory disease is known to interfere with root development in immature permanent teeth. Current research on interventions in immature teeth has been dedicated to facilitating the continuation of root development as well as regenerating the dentin–pulp complex, but the fundamental knowledge on the cellular interactions and the role of periapical mediators in apical periodontitis in immature roots that govern the disease process and post-treatment healing is limited. The limitations in 2D monolayer cell culture have a substantial role in the existing limitations of understanding cell-to-cell interactions in the pulpal and periapical tissues. Three-dimensional (3D) tissue constructs with two or more different cell populations are a better physiological representation of in vivo environment. These systems allow the high-throughput testing of multi-cell interactions and can be applied to study the interactions between stem cells and immune cells, including the role of mediators/cytokines in simulated environments. Well-designed 3D models are critical for understanding cellular functions and interactions in disease and healing processes for future therapeutic optimization in regenerative endodontics. This narrative review covers the fundamentals of (1) the disease process of apical periodontitis; (2) the influence and challenges of regeneration in immature roots; (3) the introduction of and crosstalk between mesenchymal stem cells and macrophages; (4) 3D cell culture techniques and their applications for studying cellular interactions in the pulpal and periapical tissues; (5) current investigations on cellular interactions in regenerative endodontics; and, lastly, (6) the dental–pulp organoid developed for regenerative endodontics. © 2023 by the authors.},

note = {Cited by: 10; All Open Access, Gold Open Access, Green Open Access},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Aminoshariae2022685,

title = {Insights into the June 2022 Issue of the Journal of Endodontics},

author = {Anita Aminoshariae and Amir Azarpazhooh and Ashraf F. Fouad and Gerald N. Glickman and Jianing He and Anil Kishen and Ariadne M. Letra and Linda Levin and Frank C. Setzer and Franklin R. Tay and Sahng G. Kim and Kenneth M. Hargreaves},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85134016018&doi=10.1016%2fj.joen.2022.04.013&partnerID=40&md5=795d146a97eb9cdc0b822ccc956b9afc},

doi = {10.1016/j.joen.2022.04.013},

issn = {00992399},

year  = {2022},

date = {2022-01-01},

journal = {Journal of Endodontics},

volume = {48},

number = {6},

pages = {685 – 687},

publisher = {Elsevier Inc.},

note = {Cited by: 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Li20221063,

title = {Deciphering Stem Cell From Apical Papilla–Macrophage Choreography Using a Novel 3-dimensional Organoid System},

author = {Fang-Chi Li and Hebatullah Hussein and Marco Magalhaes and P. Ravi Selvaganapathy and Anil Kishen},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85132237823&doi=10.1016%2fj.joen.2022.04.011&partnerID=40&md5=ddff2145b79797651effc0cb74b86591},

doi = {10.1016/j.joen.2022.04.011},

issn = {00992399},

year  = {2022},

date = {2022-01-01},

journal = {Journal of Endodontics},

volume = {48},

number = {8},

pages = {1063 – 1072.e7},

publisher = {Elsevier Inc.},

abstract = {Introduction: Immune cell–mesenchymal stem cell crosstalk modulates the process of repair and regeneration. In this study, a novel heterogeneous cell containing a matrix-based 3-dimensional (3D) tissue construct was used to study the interactions between stem cells from apical papilla (SCAPs) and macrophage for a comprehensive understanding on the cellular signaling mechanisms guiding inflammation and repair. Methods: SCAPs and macrophages were seeded with collagen in 3D-printed molds to generate self-assembled tissue constructs, which were exposed to 3 conditions: no stimulation, lipopolysaccharide (LPS), and interleukin (IL)-4 from 0 to 14 days. Specimens from each group were evaluated for cellular interactions, inflammatory mediators (IL-1β, tumor necrosis factor [TNF]-α, macrophage-derived chemokine [MDC], macrophage inflammatory protein [MIP]-1β, monocyte chemoattractant protein [MCP]-1, IL-6, IL-8, transforming growth factor [TGF]-β1, IL-1RA, IL-10), expression of surface markers (CD80, 206), transcription factors (pSTAT1, pSTAT6), and SCAP differentiation markers (dentin sialophosphoprotein [DSPP], dentin matrix acidic phosphoprotein 1 [DMP-1], and alizarin red) using confocal laser scanning microscopy and multiplex cytokine profiling from 2 to 14 days. Results: SCAP and macrophages displayed a cytokine-mediated interaction and differentiation characteristics. The increased pro-inflammatory cytokines/chemokines, IL-1β, TNF-α, MDC, and MIP-1β, in the earlier phase followed by the higher ratio of pSTAT6/pSTAT1 and decreased CD206 (P < .05), indicated a distinct polarization behavior in macrophages during repair in the LPS group. Conversely, the equal ratio of pSTAT6/pSTAT1, late increase in CD206, and amplified secretion of IL-1RA, IL-10, and TGF-β1 (P < .05) in the anti-inflammatory environment, directed alternative macrophage polarization, promoting SCAP differentiation and tissue modeling in IL-4 group. Conclusions: The novel 3D organoid system developed in this study allowed a comprehensive analysis of the SCAP-macrophage interactions during inflammation and healing, providing a deeper insight on the periapical dynamics of the immature tooth. © 2022 American Association of Endodontists},

note = {Cited by: 8},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Hussein2022,

title = {Application of Proteomics in Apical Periodontitis},

author = {Hebatullah Hussein and Anil Kishen},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85142928276&doi=10.3389%2ffdmed.2022.814603&partnerID=40&md5=3b716d624756ef823b89aa5d8f5ab800},

doi = {10.3389/fdmed.2022.814603},

issn = {26734915},

year  = {2022},

date = {2022-01-01},

journal = {Frontiers in Dental Medicine},

volume = {3},

publisher = {Frontiers Media S.A.},

abstract = {Apical periodontitis is an inflammatory reaction of the periradicular tissues as a consequence of multispecies microbial communities organized as biofilms within the root canal system. Periradicular tissue changes at the molecular level initiate and orchestrate the inflammatory process and precede the presentation of clinical symptoms. Inflammatory mediators have been studied at either the proteomic, metabolomic, or transcriptomic levels. Analysis at the protein level is the most common approach used to identify and quantify analytes from diseased periradicular tissues during root canal treatment, since it is more representative of definitive and active periradicular inflammatory mediator than its transcript expression level. In disease, proteins expressed in an altered manner could be utilized as biomarkers. Biomarker proteins in periradicular tissues have been qualitatively and quantitatively assessed using antibodies (immunoassays and immunostaining) or mass spectrometry-based approaches. Herein, we aim to provide a comprehensive understanding of biomarker proteins identified in clinical studies investigating periradicular lesions and pulp tissue associated with apical periodontitis using proteomics. The high throughput mass spectrometry-based proteomics has the potential to improve the current methods of monitoring inflammation while distinguishing between progressive, stable, and healing lesions for the identification of new diagnostic and therapeutic targets. This method would provide more objective tools to (a) discover biomarkers related to biological processes for better clinical case selection, and (b) determine tissue response to novel therapeutic interventions for more predictable outcomes in endodontic treatment. Copyright © 2022 Hussein and Kishen.},

note = {Cited by: 3; All Open Access, Gold Open Access},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Aminoshariae2022295,

title = {Insights into the March 2022 Issue of the JOE},

author = {Anita Aminoshariae and Amir Azarpazhooh and Anibal R. Diogenes and Ashraf F. Fouad and Gerald N. Glickman and Jianing He and Anil Kishen and Ariadne M. Letra and Linda Levin and Frank C. Setzer and Franklin R. Tay and Kenneth M. Hargreaves},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85125145718&doi=10.1016%2fj.joen.2022.01.018&partnerID=40&md5=1d73266dc7397c08942034f57c015f48},

doi = {10.1016/j.joen.2022.01.018},

issn = {00992399},

year  = {2022},

date = {2022-01-01},

journal = {Journal of Endodontics},

volume = {48},

number = {3},

pages = {295 – 297},

publisher = {Elsevier Inc.},

note = {Cited by: 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Baskar202289,

title = {Eggshell derived nano-hydroxyapatite incorporated carboxymethyl chitosan scaffold for dentine regeneration: A laboratory investigation},

author = {Kaviya Baskar and Balasubramanian Saravana Karthikeyan and Ishwarya Gurucharan and Sekar Mahalaxmi and Gurusamy Rajkumar and Vijayakumar Dhivya and Anil Kishen},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85117568391&doi=10.1111%2fiej.13644&partnerID=40&md5=75cfbf298c000d17c464e9d186701e79},

doi = {10.1111/iej.13644},

issn = {01432885},

year  = {2022},

date = {2022-01-01},

journal = {International Endodontic Journal},

volume = {55},

number = {1},

pages = {89 – 102},

publisher = {John Wiley and Sons Inc},

abstract = {Aim: To assess odontogenic differentiation abilities of porous biomineralizable composite scaffolds comprising eggshell derived nano-hydroxyapatite (HAnp) and carboxymethyl chitosan (CMC) on cultured human dental pulp stem cells (hDPSCs). Methodology: Nano-hydroxyapatite was derived from eggshells using a simple combustion method and CMC was prepared from chitosan through a chemical route. Several compositions of HAnp-CMC (0:5, 5:0, 1:5, 2:5, 3:5, 4:5 and 1:1 w/w%) scaffolds were prepared by magnetic stirring and freeze-drying methods. HAnp-CMC scaffolds were characterized using high-resolution scanning electron microscopy combined with energy-dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy and X-ray diffraction methods. In vitro bioactivity was determined following the interaction in simulated body fluid for 21 days. The optimized composite was then loaded onto hDPSCs to assess cell viability/proliferation, dentine sialophosphoprotein (DSPP) and vascular endothelial growth factor (VEGF) expressions using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, real-time quantitative polymerase chain reaction and flow cytometry methods, respectively, following 7, 14 and 21 days. For intergroup and intragroup comparisons, Kruskal–Wallis and Friedman tests were employed, respectively, followed by appropriate post hoc test (Dunn). Significant levels were set at *p <.05 and *p <.01. Results: Synthesized hydroxyapatite (HAp) comprised crystals ranging from 20 to 50 nm (HAnp) with spherulite morphology and calcium/phosphorus (Ca/P) molar ratio of 1.67. The ultrastructure of all the scaffolds revealed a highly interconnected porous microstructure, whilst the chemical characterization displayed specific functional groups of both HAnp and CMC. In vitro bioactivity assessment confirmed the biomineralization potential of all scaffolds with an apatite-like crystal formation on the surface. The 1:5 HAnp-CMC revealed a favourable pore size (60–180 µm) that was suitable for cell seeding and was chosen for further experiments. Cell viability/proliferation rates of hDPSCs loaded 1:5 HAnp-CMC at 21st day was significantly greater than that at 7th day (p <.05). The mean relative quantification of DSPP expression by the scaffold was significantly higher (p <.05) on day 21 (3.16) than on day 7 (1.67). Mean fluorescence intensity of the VEGF expression at day 21 (32.5) was also significantly higher (p <.01) than at day 7 (12.54). Conclusion: hDPSCs on 1:5 HAnp-CMC scaffolds displayed increased cell viability/proliferation and enhanced DSPP as well as VEGF expressions. The 1:5 HAnp-CMC composite has the potential to serve as a promising scaffold for dentine regeneration. © 2021 International Endodontic Journal. Published by John Wiley & Sons Ltd},

note = {Cited by: 37},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Neshatian2022,

title = {Promoting mineralization at biological interfaces Ex vivo with novel amelotin-based bio-nano complexes},

author = {Mehrnoosh Neshatian and James Holcroft and Anil Kishen and Grace De Souza and Bernhard Ganss},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85128224213&doi=10.1016%2fj.mtbio.2022.100255&partnerID=40&md5=4b457cee2a50593be2b940926fd039d1},

doi = {10.1016/j.mtbio.2022.100255},

issn = {25900064},

year  = {2022},

date = {2022-01-01},

journal = {Materials Today Bio},

volume = {14},

publisher = {Elsevier B.V.},

abstract = {Interfacial failure at the resin-dentin interface is a significant disadvantage of resin-based dental restoration. In this study, we created bio-inspired bio-nano complexes using the enamel protein amelotin (AMTN) or AMTN with an engineered collagen-binding site (AMTN-Col) to coat hydroxyapatite nanoparticles (HANP). The resulting nano-bio complexes, AMTN-HANP and AMTN-Col–HANP, were evaluated for their ability to promote collagen mineralization. Our study comprises three separate phases. In phase I, developing a method for functionalizing HANP with AMTN/AMTN-Col was explored. HANP were synthesized and characterized using TEM, SAED-TEM, XRD and ATR-FTIR. The nanoparticles were functionalized with AMTN or AMTN-Col. The successful coating of the nanoparticles with the proteins was confirmed using a TEM image of immunogold-labelled samples. In phase II of the study, the mineralization potential of the synthesized bio-nano complexes was studied using model systems consisting of simulated body fluid (SBF), polymerized collagen gels, and dentin disks prepared from human extracted molars. Mineral formation in SBF was recorded with a light scattering assay using a microplate reader on 8 replicates of each sample per study time point. Statistical analysis was performed using one-way ANOVA and the Tukey test. Significance was assigned at P ​< ​0.01. The extent of mineral formation on collagen gel and remineralization of demineralized dentin was studied with SEM. Accelerated mineral formation collagen mineralization of bio-nano complexes treated samples were observed in all model systems. In phase III of the study, the clinical utilization of AMTN/AMTN-Col coated HANP in bio-integration and enhancing the bond strength of a resin-based dental restoration and the dentin interface was investigated. The bio-nano complexes were applied as a pretreatment on dentin disks prepared from human extracted molars prior to the composite resin restoration. The micro-shear bond strength test was done on 8 samples per treatment group (a total of 32 samples). Statistical analysis on shear bond strength was performed using one-way ANOVA and the Tukey test. Significance was assigned at P ​< ​0.01. Shear bond strength values indicated that pretreatment of dentin with the bio-nano complexes before adhesive application significantly improved shear bond strength. Conclusion: We have shown that AMTN based bio-nano complexes promote mineral formation on collagenous interfaces. Our findings can be the basis of new bio-inspired, bio-nano materials that may improve dental restoration longevity by enhancing the stability and integrity of the dentin-composite resin interface. © 2022 The Authors},

note = {Cited by: 9; All Open Access, Gold Open Access, Green Open Access},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Aminoshariae20221455,

title = {Insights into the December 2022 issue of the JOE},

author = {Anita Aminoshariae and Amir Azarpazhooh and Ashraf F. Fouad and Gerald N. Glickman and Jianing He and Sahng G. Kim and Anil Kishen and Ariadne M. Letra and Linda Levin and Frank C. Setzer and Franklin R. Tay and Kenneth M. Hargreaves},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85143370628&doi=10.1016%2fj.joen.2022.11.001&partnerID=40&md5=c0046fa77a09953600b48b8bbcc99ce2},

doi = {10.1016/j.joen.2022.11.001},

issn = {00992399},

year  = {2022},

date = {2022-01-01},

journal = {Journal of Endodontics},

volume = {48},

number = {12},

pages = {1455 – 1457},

publisher = {Elsevier Inc.},

note = {Cited by: 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Hussein202277,

title = {Proteomic profiling reveals engineered chitosan nanoparticles mediated cellular crosstalk and immunomodulation for therapeutic application in apical periodontitis},

author = {Hebatullah Hussein and Anil Kishen},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85120675423&doi=10.1016%2fj.bioactmat.2021.09.032&partnerID=40&md5=7ac82f56dc42bade554479b6a653a97c},

doi = {10.1016/j.bioactmat.2021.09.032},

issn = {2452199X},

year  = {2022},

date = {2022-01-01},

journal = {Bioactive Materials},

volume = {11},

pages = {77 – 89},

publisher = {KeAi Communications Co.},

abstract = {Macrophages (MQ) are major constituents of chronically inflamed periapical tissues in apical periodontitis. This study aimed to investigate the immunomodulatory effect of engineered bioactive chitosan-based nanoparticles (CSnp) antibiofilm medication on MQ cocultured with periodontal ligament fibroblasts (PdLF). Cells viability, spreading, PdLF migration, and intracellular CSnp uptake were characterized. Tandem Mass Tag-based proteomics was applied to analyze MQ global protein expression profiles after interaction with Enterococcus faecalis biofilm, CSnp-treated biofilm, and CSnp. Secreted inflammatory mediators were analyzed. Following bioinformatics analyses, candidate proteins were validated via targeted proteomics. CSnp maintained cells viability, increased MQ spreading, and PdLF migration (p < 0.05). Transmission electron micrographs demonstrated CSnp internalization via macropinocytosis, clathrin-mediated endocytosis, and phagocytosis. Proteomic analysis revealed that CSnp-treated biofilm upregulated proteins (>1.5-folds, p < 0.05) showed functional enrichment in the pathway of metal sequestration by antimicrobial proteins, while downregulated proteins showed enrichment in ferroptosis. CSnp upregulated proteins exhibiting antioxidant and immunoregulatory properties. Upregulation of SERPINB1 by CSnp (>1.5-folds, p < 0.05) was validated. CSnp-treated biofilm reduced pro-inflammatory IL-1β and nitric oxide but enhanced anti-inflammatory IL-10 and TGF-β1 (p < 0.05). Internalized engineered bioactive CSnp reprogrammed MQ proteomic and cytokine profiles to modulate biofilm-mediated inflammation, and prompted PdLF migration, emphasizing its potential to regulate healing process in the treatment of apical periodontitis. © 2021 The Authors},

note = {Cited by: 24; All Open Access, Green Open Access},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Aminoshariae20221229,

title = {Insights into the October 2022 Issue of the JOE},

author = {Anita Aminoshariae and Amir Azarpazhooh and Ashraf F. Fouad and Gerald N. Glickman and Jianing He and Sahng G. Kim and Anil Kishen and Ariadne M. Letra and Linda Levin and Frank C. Setzer and Franklin R. Tay and Kenneth M. Hargreaves},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85138659356&doi=10.1016%2fj.joen.2022.08.007&partnerID=40&md5=0fddec84359e3abec921d2bbcbac7884},

doi = {10.1016/j.joen.2022.08.007},

issn = {00992399},

year  = {2022},

date = {2022-01-01},

journal = {Journal of Endodontics},

volume = {48},

number = {10},

pages = {1229 – 1231},

publisher = {Elsevier Inc.},

note = {Cited by: 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Aminoshariae2022837,

title = {Insights into the July 2022 Issue of the Journal of Endodontics},

author = {Anita Aminoshariae and Amir Azarpazhooh and Ashraf F. Fouad and Gerald N. Glickman and Jianing He and Sahng G. Kim and Anil Kishen and Ariadne M. Letra and Linda Levin and Frank C. Setzer and Franklin R. Tay and Kenneth M. Hargreaves},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85133188703&doi=10.1016%2fj.joen.2022.06.001&partnerID=40&md5=3dbaaa93b6ba1e53c7f242b761f72a67},

doi = {10.1016/j.joen.2022.06.001},

issn = {00992399},

year  = {2022},

date = {2022-01-01},

journal = {Journal of Endodontics},

volume = {48},

number = {7},

pages = {837 – 839},

publisher = {Elsevier Inc.},

note = {Cited by: 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Azarpazhooh202229,

title = {A Scoping Review of 4 Decades of Outcomes in Nonsurgical Root Canal Treatment, Nonsurgical Retreatment, and Apexification Studies—Part 2: Outcome Measures},

author = {Amir Azarpazhooh and Adam Sgro and Elaine Cardoso and Mohamed Elbarbary and Nima Laghapour Lighvan and Rana Badewy and Gevik Malkhassian and Hamid Jafarzadeh and Hengameh Bakhtiar and Saber Khazaei and Ariel Oren and Madeline Gerbig and Helen He and Anil Kishen and Prakesh S. Shah},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85120160661&doi=10.1016%2fj.joen.2021.09.019&partnerID=40&md5=968eb4e3d6e6d44c041b7a3bb9d0eb3e},

doi = {10.1016/j.joen.2021.09.019},

issn = {00992399},

year  = {2022},

date = {2022-01-01},

journal = {Journal of Endodontics},

volume = {48},

number = {1},

pages = {29 – 39},

publisher = {Elsevier Inc.},

abstract = {Introduction: Inconsistencies in the definitions of endodontic outcome terminology jeopardize evaluations of proposed interventions and patient care quality. This scoping review aimed to provide groundwork to develop a set of basic outcomes in endodontics. Methods: We performed a comprehensive literature search for randomized controlled trials, cohort studies, case-control studies, and case series (≥10 patients) published after 1980 with patients ≥10 years of age with any preoperative pulpal and periapical diagnosis in permanent teeth requiring nonsurgical root canal treatment, retreatment, or apexification. Abstracted data on outcome assessment methods, assessors, and domains were reported after univariate and bivariate analyses. Results: Treatment outcomes were evaluated radiographically (88%) or clinically (73%). Although 2-dimensional radiography exceeded 3-dimensional radiography, the use of the latter has increased since 2010, mostly for nonsurgical retreatments. Of 19 identified outcomes, 5 were most frequent: success (168 studies, 40%), radiographic healing (128 studies, 30%), survival (of an asymptomatic tooth [48 studies, 12%] or of a procedure code in administrative databases [31 studies, 7%]), pain assessment (14 studies, 3%), and quality of life (11 studies, 3%). Clinician-centered outcomes have been most frequently studied since the 1980s (71%), in academic settings (76%), and using a prospective design (45%). Patient-centered outcomes were reported in 19% of studies before 2010 and 30% since 2010. They were more common among retrospective studies (49%). Conclusions: Patient-centered outcome measures are lacking in endodontic studies. The state of available research can provide a baseline for the development of a core outcome set in endodontics, which should represent the important patient-centered outcomes in conjunction with well-validated clinician-centered outcomes. © 2021 The Authors},

note = {Cited by: 40; All Open Access, Hybrid Gold Open Access},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Faggion2022278,

title = {Need for criteria to appraise the methodological quality of laboratory-based studies included in systematic reviews within the speciality of Endodontology},

author = {Clovis M. Faggion and Venkateshbabu Nagendrababu and Paul V. Abbott and Christos Boutsioukis and Henry F. Duncan and Anil Kishen and Peter E. Murray and Paul M. H. Dummer},

url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-85125622086&doi=10.1111%2fiej.13700&partnerID=40&md5=b13558e2b939ec517ac1d4bd12fa56a5},

doi = {10.1111/iej.13700},

issn = {01432885},

year  = {2022},

date = {2022-01-01},

journal = {International Endodontic Journal},

volume = {55},

number = {4},

pages = {278 – 281},

publisher = {John Wiley and Sons Inc},

note = {Cited by: 0},

keywords = {},

pubstate = {published},

tppubtype = {article}

}