@article{Aminoshariae2021b,

title = {Insights into the April 2021 Issue of the Journal of Endodontics},

author = {Anita Aminoshariae and Amir Azarpazhooh and Anibal R Diogenes and Ashraf F Fouad and Gerald N Glickman and Anil Kishen and Ariadne M Letra and Linda Levin and Robert S Roda and Frank C Setzer and Franklin R Tay and Kenneth M Hargreaves},

doi = {10.1016/j.joen.2021.02.012},

issn = {1878-3554},

year  = {2021},

date = {2021-01-01},

journal = {Journal of Endodontics},

volume = {47},

issue = {4},

pages = {555-557},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Aminoshariae2021c,

title = {Insights into the May 2021 Issue of the JOE},

author = {Anita Aminoshariae and Amir Azarpazhooh and Anibal R Diogenes and Ashraf F Fouad and Gerald N Glickman and Anil Kishen and Ariadne M Letra and Linda Levin and Robert S Roda and Frank C Setzer and Franklin R Tay and Kenneth M Hargreaves},

doi = {10.1016/j.joen.2021.03.013},

issn = {1878-3554},

year  = {2021},

date = {2021-01-01},

journal = {Journal of Endodontics},

volume = {47},

issue = {5},

pages = {681-683},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Suresh2021,

title = {Maxillary Anterior Teeth With Extensive Root Resorption Treated With Low-level Light-activated Engineered Chitosan Nanoparticles},

author = {Nandini Suresh and Haritha Jamuna Subbarao and Velmurugan Natanasabapathy and Anil Kishen},

doi = {10.1016/j.joen.2021.04.014},

issn = {1878-3554},

year  = {2021},

date = {2021-01-01},

journal = {Journal of Endodontics},

volume = {47},

issue = {7},

pages = {1182-1190},

abstract = {INTRODUCTION: Photoactivated chitosan-based nanoparticles can eliminate bacterial biofilm, inactivate endotoxins, improve resistance to biological degradation (resorption), and promote bone regeneration. This case is the first documentation to highlight the successful healing of teeth with extensive inflammatory root resorption (IRR) with periapical lesions using a combined surgical and nonsurgical root canal therapy using rose bengal functionalized chitosan nanoparticles (CSRBnp). 

METHODS: A 17-year-old boy with extensive IRR of maxillary right canine (teeth #6), maxillary right lateral incisor (#7), maxillary right central incisor (#8), and maxillary left central incisor (#9) was treated with photoactivated CSRBnp, both intracanal as well as topically on resorptive defects and periapical lesions. The larger external resorptive defects on the root surfaces were restored with Biodentine, whereas the through-and-through periapical lesions were packed with sticky bone for Guided Bone Regeneration. 

RESULTS: At 26 months of follow-up, the clinical, 2-dimensional (intraoral periapical radiographs) and 3-dimensional (cone-beam computed tomography) images showed absence of clinical symptoms, teeth mobility, arrested IRR, and significant osseous healing of the periradicular region. Postoperatively, the patient retraumatized thrice in relation to #7 resulting in horizontal root fracture, which showed type I pattern of root fracture healing in the follow-up. 

CONCLUSIONS: Photoactivated chitosan-based nanoparticles can be a viable therapeutic option to hinder root resorption while enhancing healing outcomes in cases of severe IRR.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Aminoshariae2021d,

title = {Insights into the June 2021 Issue of the JOE},

author = {Anita Aminoshariae and Amir Azarpazhooh and Anibal R Diogenes and Ashraf F Fouad and Gerald N Glickman and Anil Kishen and Ariadne M Letra and Linda Levin and Robert S Roda and Frank C Setzer and Franklin R Tay and Kenneth M Hargreaves},

doi = {10.1016/j.joen.2021.05.001},

issn = {1878-3554},

year  = {2021},

date = {2021-01-01},

journal = {Journal of Endodontics},

volume = {47},

issue = {6},

pages = {849-851},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Aminoshariae2021e,

title = {Insights into the July 2021 Issue of the Journal of Endodontics},

author = {Anita Aminoshariae and Amir Azarpazhooh and Anibal R Diogenes and Ashraf F Fouad and Gerald N Glickman and Anil Kishen and Ariadne M Letra and Linda Levin and Robert S Roda and Frank C Setzer and Franklin R Tay and Kenneth M Hargreaves},

doi = {10.1016/j.joen.2021.05.009},

issn = {1878-3554},

year  = {2021},

date = {2021-01-01},

journal = {Journal of Endodontics},

volume = {47},

issue = {7},

pages = {1043-1045},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Hussein2021,

title = {Engineered Chitosan-based Nanoparticles Modulate Macrophage-Periodontal Ligament Fibroblast Interactions in Biofilm-mediated Inflammation},

author = {Hebatullah Hussein and Anil Kishen},

doi = {10.1016/j.joen.2021.06.017},

issn = {1878-3554},

year  = {2021},

date = {2021-01-01},

journal = {Journal of Endodontics},

volume = {47},

issue = {9},

pages = {1435-1444},

abstract = {INTRODUCTION: Crosstalk between immune cells and tissue-resident cells regulates the pathophysiology and posttreatment healing of apical periodontitis. This investigation aimed to understand the influence of residual root canal biofilm on macrophage (MQ)-periodontal ligament fibroblast (PdLF) interaction and evaluate the effect of engineered chitosan-based nanoparticles (CSnp) on MQ-PdLF interactions in residual biofilm-mediated inflammation. METHODS: Six-week-old Enterococcus faecalis biofilms in root canal models were disinfected conventionally using sodium hypochlorite alone or followed by calcium hydroxide medication or CSnp dispersed in carboxymethylated chitosan (CMCS). The effect of the treated biofilms (n = 25/group) on the inflammatory response of THP-1-differentiated MQ monoculture versus coculture with PdLF was evaluated for cell viability, MQ morphometric characterization, inflammatory mediators (nitric oxide, tumor necrosis factor alpha, interleukin [IL]-1 beta, IL-1RA, IL-6, transforming growth factor beta 1 [TGF-β1], and IL-10), and the expression of transcription factors (pSTAT1/pSTAT6)/cluster of differentiation markers (CD80/206) after 24, 48, and 72 hours of interaction. PdLF transwell migration was evaluated after 8 and 24 hours. Unstimulated cells served as the negative control, whereas untreated biofilm was the positive control. 

RESULTS: Biofilm increased nitric oxide and IL-1β but suppressed IL-10, IL-1RA, and PdLF migration with significant cytotoxic effects. CSnp/CMCS reduced nitric oxide and IL-1β (P .01) while maintaining ≥90% cell survival up to 72 hours with evident M2-like MQ phenotypic changes in coculture. CSnp/CMCS also increased the IL-1RA/IL-1β ratio and enhanced TGF-β1 production over time (P .05, 72 hours). In coculture, CSnp/CMCS showed the highest IL-10 level at 72 hours (P .01), reduced the pSTAT1/pSTAT6 ratio, and enhanced PdLF migration (P .01, 24 hours). 

CONCLUSIONS: CSnp/CMCS medication facilitated MQ switch toward M2 (regulatory/anti-inflammatory) phenotype and PdLF migration via paracrine signaling.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Aminoshariae2021f,

title = {Insights into the August 2021 Issue of the JOE},

author = {Anita Aminoshariae and Amir Azarpazhooh and Anibal R Diogenes and Ashraf F Fouad and Gerald N Glickman and Anil Kishen and Ariadne M Letra and Linda Levin and Robert S Roda and Frank C Setzer and Franklin R Tay and Kenneth M Hargreaves},

doi = {10.1016/j.joen.2021.06.016},

issn = {1878-3554},

year  = {2021},

date = {2021-01-01},

journal = {Journal of Endodontics},

volume = {47},

issue = {8},

pages = {1195-1197},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Marashdeh2021b,

title = {Assessment of Root Canal Sealers Loaded with Drug-Silica Coassembled Particles Using an In Vitro Tooth Model},

author = {Muna Q Marashdeh and Cameron A Stewart and Anil Kishen and Celine Levesque and Yoav Finer},

doi = {10.1016/j.joen.2021.08.005},

issn = {1878-3554},

year  = {2021},

date = {2021-01-01},

journal = {Journal of Endodontics},

volume = {47},

issue = {11},

pages = {1775-1782},

abstract = {INTRODUCTION: The purpose of this study was to assess the antimicrobial activity of root canal sealers modified with novel highly loaded antimicrobial drug-silica coassembled particles (DSPs) on Enterococcus faecalis-infected root canal dentin. 

METHODS: DSPs were synthesized through coassembly of silica and octenidine dihydrochloride (OCT) surfactant drug (35% w/w OCT). DSPs (1% wt of the total mass of the sealer) were mixed homogenously with either epoxy resin sealer (AH Plus [AH]; Dentsply Sirona, Tulsa, OK) or calcium silicate-based sealer (EndoSequence BC Sealer [BC]; Brasseler, Savannah, GA). To assess the antimicrobial activity of DSP-loaded sealers, the apical third of single-rooted teeth was obtained and infected with E. faecalis for 3 weeks followed by the application of experimental (DSP-loaded) sealers or corresponding controls for up to 28 days. Microbiological analysis and laser scanning confocal and scanning electron microscopy were used to determine the colony-forming unit (CFU)/mL, the percentage of live bacteria, and the intratubular bacterial and sealer penetrations. Factorial analysis of variance and Tukey post hoc tests were used to assess the antimicrobial effect of DSPs on different sealers. 

RESULTS: All experimental groups showed significant reductions in CFUs at all-time points compared with positive controls (P .05). The addition of DSPs to BC significantly reduced the CFUs (2.11 ± 0.13, 2.22 ± 0.19, and 2.25 ± 0.17 at 1, 7, and 28 days, respectively) compared with the unmodified sealer (3.21 ± 0.11, 4.3 ± 0.15, and 4.2 ± 0.2 at 0, 7, and 28 days). DSPs enhanced the antimicrobial performance of AH only at 1 day (4.21 ± 0.17 vs 5.19 ± 0.12, P .05). AH and AH + DSPs showed higher bacterial viability compared with BC and BC + DSPs at all incubation periods (P .05). 

CONCLUSIONS: Loading endodontic sealers with DSPs had a material-dependent effect on the antimicrobial properties and could reduce the incidence of secondary infections.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Aminoshariae2021g,

title = {Insights into the September 2021 Issue of the JOE},

author = {Anita Aminoshariae and Amir Azarpazhooh and Anibal R Diogenes and Ashraf F Fouad and Gerald N Glickman and Anil Kishen and Ariadne M Letra and Linda Levin and Robert S Roda and Frank C Setzer and Franklin R Tay and Kenneth M Hargreaves},

doi = {10.1016/j.joen.2021.07.019},

issn = {1878-3554},

year  = {2021},

date = {2021-01-01},

journal = {Journal of Endodontics},

volume = {47},

issue = {9},

pages = {1337-1339},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Aminoshariae2021h,

title = {Insights into the October 2021 Issue of the Journal of Endodontics},

author = {Anita Aminoshariae and Amir Azarpazhooh and Anibal R Diogenes and Ashraf F Fouad and Gerald N Glickman and Anil Kishen and Ariadne M Letra and Linda Levin and Robert S Roda and Frank C Setzer and Franklin R Tay and Kenneth M Hargreaves},

doi = {10.1016/j.joen.2021.08.011},

issn = {1878-3554},

year  = {2021},

date = {2021-01-01},

journal = {Journal of Endodontics},

volume = {47},

issue = {10},

pages = {1547-1549},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Aminoshariae2021i,

title = {Insights into the November 2021 Issue of the Journal of Endodontics},

author = {Anita Aminoshariae and Amir Azarpazhooh and Anibal R Diogenes and Ashraf F Fouad and Gerald N Glickman and Anil Kishen and Ariadne M Letra and Linda Levin and Robert S Roda and Frank C Setzer and Franklin R Tay and Kenneth M Hargreaves},

doi = {10.1016/j.joen.2021.09.013},

issn = {1878-3554},

year  = {2021},

date = {2021-01-01},

journal = {Journal of Endodontics},

volume = {47},

issue = {11},

pages = {1669-1671},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Aminoshariae2021j,

title = {Insights into the December 2021 Issue of the JOE},

author = {Anita Aminoshariae and Amir Azarpazhooh and Anibal R Diogenes and Ashraf F Fouad and Gerald N Glickman and Jianing He and Anil Kishen and Ariadne M Letra and Linda Levin and Robert S Roda and Frank C Setzer and Franklin R Tay and Kenneth M Hargreaves},

doi = {10.1016/j.joen.2021.10.007},

issn = {1878-3554},

year  = {2021},

date = {2021-01-01},

journal = {Journal of Endodontics},

volume = {47},

issue = {12},

pages = {1817-1819},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Ozlek2020,

title = {A chitosan-based irrigant improves the dislocation resistance of a mineral trioxide aggregate-resin hybrid root canal sealer},

author = {Esin Ozlek and Priti Pragati Rath and Anil Kishen and Prasanna Neelakantan},

doi = {10.1007/s00784-019-02916-x},

issn = {1436-3771},

year  = {2020},

date = {2020-01-01},

journal = {Clinical Oral Investigations},

volume = {24},

issue = {1},

pages = {151-156},

abstract = {OBJECTIVES: To investigate the effectiveness of root canal irrigation with chitosan on the dislocation resistance of a root canal sealer (MTA Fillapex) in vitro, measured by the push-out bond strength test. MATERIALS AND METHODS: Root canals of mandibular premolars (n = 57) were prepared using rotary files with 5.25% sodium hypochlorite as the irrigant during instrumentation. Following this, the specimens were randomly divided into three groups (n = 19) based on the final irrigant: group 1, 0.2% chitosan solution; group 2, 17% EDTA solution; group 3, saline. Three specimens from each group were analyzed using scanning electron microscopy-energy dispersive spectroscopy (SEM-EDS). The remaining specimens of each group were divided into two subgroups (n = 8) based on the method of agitation of the final irrigants (chitosan/EDTA/saline): subgroup A, sonic (Endoactivator, Dentsply Maillefer); subgroup B, no activation (control). After irrigation, all specimens obturated with a commercial mineral trioxide aggregate-resin hybrid sealer (MTA Fillapex, Angelus, Londrina, Brazil). Dislocation resistance was measured using the push-out bond strength test after 3 weeks. The data were analyzed using Kruskal-Wallis test (P = 0.05). 

RESULTS: Immaterial of the irrigant agitation, groups irrigated with chitosan showed significantly higher bond strength values than those irrigated with EDTA (P 0.05). Groups irrigated with saline showed the least bond strength values (P 0.05). When EDTA was used, sonic agitation significantly improved the bond strength of the sealer, compared to the control (P 0.05). There was no significant difference between sonic agitation and the control when chitosan solution was used as the final irrigant (P 0.05). The nitrogen/carbon ratio was significantly higher in chitosan groups compared to the control group (P 0.05). 

CONCLUSION: This study provides the first evidence that chitosan irrigation improves the dislocation resistance of MTA-resin hybrid root canal sealer, compared to EDTA and saline irrigation. 

CLINICAL RELEVANCE: Chitosan-based irrigation has been previously shown to demonstrate anti-biofilm properties in the root canal. The present study demonstrates that chitosan can improve the bond strength of a root filling material, which may contribute to better sealing of the root canal system.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Bakhtiar2020,

title = {Pulp ECM-derived macroporous scaffolds for stimulation of dental-pulp regeneration process},

author = {Hengameh Bakhtiar and Mohammad Pezeshki-Modaress and Zahra Kiaipour and Mahdieh Shafiee and Mohammad Reza Ellini and Amir Mazidi and Sarah Rajabi and Soheila Zamanlui Benisi and Seyed Naser Ostad and Kerstin Galler and Pardis Pakshir and Amir Azarpazhooh and Anil Kishen},

doi = {10.1016/j.dental.2019.10.011},

issn = {1879-0097},

year  = {2020},

date = {2020-01-01},

journal = {Dental Materials: Official Publication of the Academy of Dental Materials},

volume = {36},

issue = {1},

pages = {76-87},

abstract = {OBJECTIVE: Recent studies suggest xenogeneic extracellular matrices as potential regenerative tools in dental pulp regeneration. This study aimed to fabricate and characterize a novel three-dimensional macroporous pulp-derived scaffold that enables the attachment, penetration, proliferation and differentiation of mesenchymal stem cells. 

METHOD: Bovine pulp was decellularized and characterized with histological and DNA content methods. This scaffold was prepared using finely milled lyophilized decellularized pulp extracellular matrix (ECM) digested with pepsin. Three different concentrations of ECM (1.50, 2.25 and 3.00mg/ml) were freeze-dried and were tested with/without chemical crosslinking. The specimens were subjected to physicochemical characterization, cell viability and quantitative real time polymerase chain reaction assessments with human bone marrow mesenchymal stem cells (hBMMSCs). All scaffolds were subcutaneously implanted in rats for two weeks and histological and immunostaining analyses were performed. 

RESULTS: Histological and DNA analysis confirmed complete decellularization. All samples demonstrated more than 97% porosity and 1.50mg/ml scaffold demonstrated highest water absorption. The highest cell viability and proliferation of hBMMSCs was observed on the 3.00mg/ml crosslinked scaffolds. The gene expression analysis showed a significant increase of dmp-1 and collagen-I on 3.00mg/ml crosslinked scaffolds compared to the other scaffolds. Histological examination of subcutaneous implanted scaffolds revealed low immunological response, and enhanced angiogenesis in cross-linked samples compared to non-crosslinked samples. 

SIGNIFICANCE: The three-dimensional macroporous pulp-derived injectable scaffold developed and characterized in this study displayed potential for regenerative therapy. While the scaffold biodegradability was decreased by crosslinking, the biocompatibility of post-crosslinked scaffold was significantly improved.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Bakhtiar2020b,

title = {Potential of Treated Dentin Matrix Xenograft for Dentin-Pulp Tissue Engineering},

author = {Hengameh Bakhtiar and Amir Mazidi and Saeed Mohammadi-Asl and Sadegh Hasannia and Mohammad Reza Ellini and Mohammad Pezeshki-Modaress and Seyed Naser Ostad and Kerstin Galler and Amir Azarpazhooh and Anil Kishen},

doi = {10.1016/j.joen.2019.10.005},

issn = {1878-3554},

year  = {2020},

date = {2020-01-01},

journal = {Journal of Endodontics},

volume = {46},

issue = {1},

pages = {57\textendash64.e1},

abstract = {INTRODUCTION: This study aims to develop and characterize the regenerative potential of an atelopeptidized treated dentin matrix xenograft using in vitro and in vivo models. 

METHODS: Freshly extracted bovine dentin was pulverized into 250- to 500-μm particles and demineralized with 17% EDTA for 1, 7, and 13 days. The samples were atelopeptidized with pepsin. The degree of demineralization and the effect of atelopeptidization were assessed using field emission scanning electron microscopy combined with energy-dispersive X-ray spectroscopy and Fourier transform infrared spectroscopy, respectively. The expression of dentin matrix acidic phosphoprotein 1, dentin sialophosphoprotein, and osteopontin was evaluated in dental pulp stem cells using quantitative real-time polymerase chain reaction. The samples were then implanted intramuscularly in rats for 30 days, and the inflammatory cells were quantified histologically. 

RESULTS: Field emission scanning electron microscopy combined with energy-dispersive X-ray spectroscopy revealed an exposed tubular structure of dentin after 1 and 7 days of demineralization. Fourier transform infrared spectroscopy confirmed the absence of amide peaks at 1260 to 1640/cm after atelopeptidization. The dental pulp stem cell expression of dentin matrix acidic phosphoprotein 1 and dentin sialophosphoprotein increased in all compared with the untreated control group (P .05). The maximum expression rates were observed for the 1-day demineralized and atelopeptidized group. The 1-day demineralized group elicited the highest inflammatory response compared with the 7- or 13-day demineralized groups (P .001). Atelopeptidization significantly decreased the inflammatory response only in the 1-day demineralized dentin group (P .05). 

CONCLUSIONS: Atelopeptidization of 1-day demineralized dentin xenograft preserved the collagen structure, minimized the immune reaction, and provided sufficient regenerative potential.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Azarpazhooh2020,

title = {Insights into the January 2020 Issue of the Journal of Endodontics},

author = {Amir Azarpazhooh and Anibal R Diogenes and Ashraf F Fouad and Gerald N Glickman and Mo K Kang and Anil Kishen and Linda Levin and Robert S Roda and Christine M Sedgley and Franklin R Tay and Kenneth M Hargreaves},

doi = {10.1016/j.joen.2019.11.008},

issn = {1878-3554},

year  = {2020},

date = {2020-01-01},

journal = {Journal of Endodontics},

volume = {46},

issue = {1},

pages = {1-2},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Thang2020,

title = {The effects of physical photostimulable phosphor plate artifacts on the radiologic interpretation of periapical inflammatory disease},

author = {Trevor S T Thang and Anil Kishen and Massieh Moayedi and Pascal N Tyrrell and Wenda Zhao and Susanne E Perschbacher},

doi = {10.1016/j.oooo.2019.11.001},

issn = {2212-4411},

year  = {2020},

date = {2020-01-01},

journal = {Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology},

volume = {129},

issue = {6},

pages = {621-628},

abstract = {OBJECTIVE: To evaluate how physical photostimulable phosphor (PSP) plate artifacts, such as those created by scratches, phosphor degradation, and surface peeling, affect the radiologic interpretation of periapical inflammatory disease. 

STUDY DESIGN: A novel technique was developed to digitally superimpose 25 real PSP artifact masks over 100 clinical complementary metal oxide semiconductor (CMOS) periapical images with known radiologic interpretations. These images were presented to 25 general dentists, who were asked to state their radiologic interpretations, their confidence in their interpretations, and their opinions on whether the plates should be discarded. Statistical analyses were conducted by using random intercept mixed models for repeated measures and χ2 tests of the pooled data. 

RESULTS: No statistically significant adverse effect on interpretation was seen, even at severe artifact levels. There was a statistically significant decrease in the clinicians' confidence and an increase in discard proportions when interpreting images with severe PSP plate artifacts (P .05). 

CONCLUSIONS: Although diagnostic efficacy was unaffected, clinicians' confidence decreased and proportionally more clinicians opted to discard sensors when interpreting images with severe artifacts. Future studies on the effects of artifacts on the efficacy of diagnosis of other dental diseases are recommended. Ultimately, these results can guide recommendations for PSP plate quality assurance.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Azarpazhooh2020b,

title = {Insights into the February 2020 Issue of the Journal of Endodontics},

author = {Amir Azarpazhooh and Anibal R Diogenes and Ashraf F Fouad and Gerald N Glickman and Mo K Kang and Anil Kishen and Linda Levin and Robert S Roda and Christine M Sedgley and Franklin R Tay and Kenneth M Hargreaves},

doi = {10.1016/j.joen.2020.01.001},

issn = {1878-3554},

year  = {2020},

date = {2020-01-01},

journal = {Journal of Endodontics},

volume = {46},

issue = {2},

pages = {147-148},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Azarpazhooh2020c,

title = {Insights into the March 2020 Issue of the Journal of Endodontics},

author = {Amir Azarpazhooh and Anibal R Diogenes and Ashraf F Fouad and Gerald N Glickman and Mo K Kang and Anil Kishen and Linda Levin and Robert S Roda and Christine M Sedgley and Franklin R Tay and Kenneth M Hargreaves},

doi = {10.1016/j.joen.2020.02.001},

issn = {1878-3554},

year  = {2020},

date = {2020-01-01},

journal = {Journal of Endodontics},

volume = {46},

issue = {3},

pages = {343-344},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Kukreti2020,

title = {Efficacy of bioactive nanoparticles on tissue-endotoxin induced suppression of stem cell viability, migration and differentiation},

author = {H Kukreti and F. -C. Li and K Singh and R Sodhi and A Kishen},

doi = {10.1111/iej.13283},

issn = {1365-2591},

year  = {2020},

date = {2020-01-01},

journal = {International Endodontic Journal},

volume = {53},

issue = {6},

pages = {859-870},

abstract = {AIM: To characterize a lipopolysaccharide (LPS)-treated dentine tissue model (LPS dentine) to analyse the efficacy of polycationic chitosan nanoparticles (CSnp) and/or dexamethasone conjugate chitosan nanoparticles (Dex-CSnp) on the viability/differentiation potential of stem cells from apical papilla (SCAP) when exposed to LPS dentine. A further aim was to understand the effect of macrophage-dependent inflammation on SCAP migration in the presence of LPS dentine. METHODOLOGY: A total of 88 dentine slabs were used. TOF-SIMS analysis was performed amongst the LPS-treated and untreated dentine groups (n = 2/group). The study was conducted using four dentine groups: no treatment (control); LPS treatment only; LPS treatment followed by CSnp conditioning; and LPS treatment followed by Dex-CSnp conditioning groups. SCAP adherence, viability, differentiation and biomineralization potential on dentine from different groups were studied using fluorescent and scanning electron microscopy. Inflammation by macrophages in response to LPS dentine was quantified, and effect on SCAP migration was analysed. Statistical analysis was performed using Student's t-test with a significance level of P 0.05. 

RESULT: TOF-SIMS analysis confirmed LPS contamination. LPS dentine affected SCAP viability but not adherence to dentine (P 0.001). Conditioning of LPS dentine with either nanoparticles improved SCAP viability (P 0.01) and rescued other LPS related adverse effects on SCAPs, such as F-actin disruption, decrease in differentiation/biomineralization potential. IL-6 produced by macrophages in response to LPS-treated dentine impeded SCAP migration (P 0.001), diminished on CSnp and Dex-CSnp conditioning groups (P 0.01). 

CONCLUSION: This study developed an LPS-dentine model and highlighted the ability of CSnp and Dex-CSnp to promote stem cell viability, migration, differentiation potential and reduce inflammation, providing an environment conducive for tissue regeneration/repair.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}